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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Overview
Product name
Anti-TRAP220/MED1 antibody
See all TRAP220/MED1 products (8) ...
Description
Rabbit polyclonal to TRAP220/MED1
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Orangutan
Immunogen
Synthetic peptide: HKKEKKKVKD KDRDRDRDKD RDKKKSHSIK PESWSKSPIS SDQSLSMTSN TILSADRPSR LSPDFMIGEE DDDLMDVALI GN, corresponding to C terminal amino acids 1500-1581 of Human TRAP220/MED1
HKKEKKKVKD KDRDRDRDKD RDKKKSHSIK PESWSKSPIS SDQSLSMTSN TILSADRPSR LSPDFMIGEE DDDLMDVALI GN
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 1% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/2500.Predicted molecular weight: 180 kDa.
IP: Use at an assay dependent dilution.
Target
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the Mediator complex subunit 1 family.
Post-translational
modifications
Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
Target information above from: UniProt accessionQ15648
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activator-recruited cofactor 205 kDa component antibody
- ARC205 antibody
- CRSP1 antibody
see all
Database links
- Entrez Gene: 5469 Human
- Entrez Gene: 19014 Mouse
- Omim: 604311 Human
- SwissProt: Q15648 Human
- SwissProt: Q6P9H7 Human
- SwissProt: O88323 Mouse
- SwissProt: Q3UHV0 Mouse
- SwissProt: Q6AXD5 Mouse
see all
References for Anti-TRAP220/MED1 antibody (ab52065)
ab52065 has not yet been referenced specifically in any publications.
Publishing research using ab52065? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"