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Immunocytochemistry/ Immunofluorescence - TRAP220/MED1 antibody (ab61119)
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Product Name
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TRAP220/MED1 antibody
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See all TRAP220/MED1 antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to TRAP220/MED1
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Immunogen
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Synthetic non-phosphopeptide derived from human TRAP220/MED1 around the phosphorylation site of threonine 1457.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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ab61119 detects endogenous levels of total TRAP220/MED1 protein.
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Tested applications
(see key)
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ELISA, ICC/IF
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/10000.
ICC/IF: 1/500 - 1/1000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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HeLa cells.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
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Relevance
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TRAP220/MED1 work with coactivators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53 dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize (referenced from Entrez Gene).
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TRAP220/MED1 antibody (ab61119) |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Purification notes
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ab61119 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at -20°C. Stable for 12 months at -20°C
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this TRAP220/MED1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for TRAP220/MED1 antibody images, references, products related to ab61119 and other tools.
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TRAP220/MED1 antibody images:
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 Immunocytochemistry/ Immunofluorescence - TRAP220/MED1 antibody (ab61119)
ab61119, at a 1/50 dilution, staining human TRAP220/MED1 in HeLa cells with (+) or without (-) immunising peptide, using Immunofluorescence
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Search PubMed (MEDLINE) for references to TRAP220/MED1
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TRAP220/MED1 antibody - more information
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Customer reviews (feedback) regarding TRAP220/MED1 antibody |
Customer FAQs regarding TRAP220/MED1 antibody |
Protocols for TRAP220/MED1 antibody |
Price and availability of products related to TRAP220/MED1 antibody |
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TRAP220/MED1 antibody - related products:
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TRAP220/MED1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about TRAP220/MED1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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