Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Overview
Product name
Anti-TRAP220/MED1 antibody
See all TRAP220/MED1 products (8) ...
Description
Rabbit polyclonal to TRAP220/MED1
Specificity
ab61119 detects endogenous levels of total TRAP220/MED1 protein.
Tested applications
ICC/IF, ELISAmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic non-phosphopeptide derived from human TRAP220/MED1 around the phosphorylation site of threonine 1457.
Positive control
HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab61119 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
-
Immunocytochemistry/ Immunofluorescence - TRAP220/MED1 antibody (ab61119)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab61119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/500 - 1/1000.
ELISA
ELISA: 1/10000ELISA: 1/10000
Target
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the Mediator complex subunit 1 family.
Post-translational
modifications
Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
Target information above from: UniProt accessionQ15648
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activator-recruited cofactor 205 kDa component antibody
- ARC205 antibody
- CRSP1 antibody
see all
Database links
- Entrez Gene: 5469 Human
- Entrez Gene: 19014 Mouse
- Omim: 604311 Human
- SwissProt: Q15648 Human
- SwissProt: Q6P9H7 Human
- SwissProt: O88323 Mouse
- SwissProt: Q3UHV0 Mouse
- SwissProt: Q6AXD5 Mouse
see all
Anti-TRAP220/MED1 antibody images:
Immunocytochemistry/ Immunofluorescence - TRAP220/MED1 antibody (ab61119)
ab61119, at a 1/50 dilution, staining human TRAP220/MED1 in HeLa cells with (+) or without (-) immunising peptide, using Immunofluorescence
References for Anti-TRAP220/MED1 antibody (ab61119)
ab61119 has not yet been referenced specifically in any publications.
Publishing research using ab61119? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"