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Reassurance, Refunds & Replacements
If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »Publishing research using ab64965? Please let us know so that we can cite the reference in this datasheet
ab64965 has been referenced in 2 publications.
- Mouillet JF et al. MiR-205 silences MED1 in hypoxic primary human trophoblasts. FASEB J 24:2030-9 (2010). WB; Human. PubMed: 20065103
- Heyd F & Lynch KW Phosphorylation-dependent regulation of PSF by GSK3 controls CD45 alternative splicing. Mol Cell 40:126-37 (2010). WB; Human. PubMed: 20932480
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Western blot - TRAP220/MED1 antibody (ab64965)
All lanes : Anti-TRAP220/MED1 antibody (ab64965) at 1/500 dilution
Lane 1 : extracts from Jurkat cells, without immunising peptide
Lane 2 : extracts from Jurkat cells, with immunising peptide
Predicted band size : 180 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TRAP220/MED1 antibody (ab64965)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using 1/50 ab64965, after antigen retrieval by boiling in a pressure cooker for 2-3 min. Polymer system was used for signal enhancing and DAB staining was performed for visualising the signal.
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