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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - TRAP220/MED1 antibody (ab64965)
All lanes : Anti-TRAP220/MED1 antibody (ab64965) at 1/500 dilution
Lane 1 : extracts from Jurkat cells, without immunising peptide
Lane 2 : extracts from Jurkat cells, with immunising peptide
Predicted band size : 180 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TRAP220/MED1 antibody (ab64965)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using 1/50 ab64965, after antigen retrieval by boiling in a pressure cooker for 2-3 min. Polymer system was used for signal enhancing and DAB staining was performed for visualising the signal.
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