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Products:Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Overview
Product name
Anti-TRAP220/MED1 antibody
See all TRAP220/MED1 products (8) ...
Description
Rabbit polyclonal to TRAP220/MED1
Tested applications
ELISA, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide derived from internal region of human TRAP220/MED1
Positive control
Human breast carcinoma tissue. Extracts from Jurkat cells.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Epigenetics and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab64965 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/10000ELISA: 1/10000
IHC-P: 1/50 - 1/100.Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB: 1/500 - 1/1000.Detects a band of approximately 170 kDa (predicted molecular weight: 180 kDa).
Target
Function
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Tissue specificity
Ubiquitously expressed.
Sequence similarities
Belongs to the Mediator complex subunit 1 family.
Post-translational
modifications
Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
Target information above from: UniProt accessionQ15648
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activator-recruited cofactor 205 kDa component antibody
- ARC205 antibody
- CRSP1 antibody
see all
Database links
- Entrez Gene: 5469 Human
- Entrez Gene: 19014 Mouse
- Omim: 604311 Human
- SwissProt: Q15648 Human
- SwissProt: Q6P9H7 Human
- SwissProt: O88323 Mouse
- SwissProt: Q3UHV0 Mouse
- SwissProt: Q6AXD5 Mouse
see all
Anti-TRAP220/MED1 antibody images:
Western blot - TRAP220/MED1 antibody (ab64965)
All lanes : Anti-TRAP220/MED1 antibody (ab64965) at 1/500 dilution
Lane 1 : extracts from Jurkat cells, without immunising peptide
Lane 2 : extracts from Jurkat cells, with immunising peptide
Predicted band size : 180 kDa
Observed band size : 170 kDa (why is the actual band size different from the predicted?)
Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TRAP220/MED1 antibody (ab64965)
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using 1/50 ab64965, after antigen retrieval by boiling in a pressure cooker for 2-3 min. Polymer system was used for signal enhancing and DAB staining was performed for visualising the signal.
References for Anti-TRAP220/MED1 antibody (ab64965)
This product has been referenced in:
- Mouillet JFet al. MiR-205 silences MED1 in hypoxic primary human trophoblasts. FASEB J 24:2030-9 (2010). WB; Human.Read more (PubMed: 20065103) »
- Heyd F & Lynch KW Phosphorylation-dependent regulation of PSF by GSK3 controls CD45 alternative splicing. Mol Cell 40:126-37 (2010). WB; Human.Read more (PubMed: 20932480) »
See all 2 publications for this product
Publishing research using ab64965? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"