TRAP220/MED1 (phospho T1457) antibody - ChIP Grade (ab60950)

Product Name 

TRAP220/MED1 (phospho T1457) antibody - ChIP Grade

Product type 

Primary antibodies

Description 

Rabbit polyclonal to TRAP220/MED1 (phospho T1457) - ChIP Grade

Immunogen 

Synthetic phosphopeptide derived from human TRAP220/MED1 around the phosphorylation site of threonine 1457 (A-Y-T^P-P-Q).

Reacts with 
(species key)

Hu, Ms

Specificity 

ab60950 detects endogenous levels of TRAP220/MED1 only when phosphorylated at threonine 1457.

Tested applications 
(see key)

ChIP, ELISA, ICC/IF, IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
ChIP: Use at an assay dependent dilution (PMID 19122196).
ELISA: 1/5000.
ICC/IF: 1/500 - 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 180 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human heart tissue, HeLa cells and HuvEc cell extracts

Cellular localization 

Nuclear

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Chromatin and Nuclear Signaling >> Transcription >> Polymerase associated factors >> Pol II Transcription >> Other

Relevance 

TRAP220/MED1 work with coactivators to direct transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor-(TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. It also regulates p53 dependent apoptosis and it is essential for adipogenesis. This protein is known to have the ability to self-oligomerize (referenced from Entrez Gene).

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Purification notes 

ab60950 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

See below for TRAP220/MED1 (phospho T1457) antibody - ChIP Grade images, references, products related to ab60950 and other tools.

TRAP220/MED1 (phospho T1457) antibody - ChIP Grade images:

References for TRAP220/MED1 (phospho T1457) antibody - ChIP Grade (ab60950)

  This product has been used in:

Saramki A et al. Cyclical Chromatin Looping and Transcription Factor Association on the Regulatory Regions of the p21 (CDKN1A) Gene in Response to 1{alpha},25-Dihydroxyvitamin D3. J Biol Chem 284:8073-82 (2009). ChIP; Human. PubMed: 19122196

If you publish research using ab60950 please let us know so that we can cite the reference on this datasheet.

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