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Anti-TRBP antibody (ab72547)

Abreviews (2)Q&A (5)

Overview

  • Product nameAnti-TRBP antibodySee all TRBP primary antibodies ...
  • Description
    Rabbit polyclonal to TRBP
  • Tested applicationsICC/IF, WB, IHC-P more details
  • Species reactivity
    Reacts with: Horse, Human, Monkey
    Predicted to work with: Mouse, Rat, Chicken
  • Immunogen

    Synthetic peptide corresponding to a region between residues 340-390 of human TRBP (NP_599150.1)

  • Positive controlHuman brain lysate and human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab72547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: Use at an assay dependent concentration.
WB WB: Use a concentration of 5 - 8 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa).
IHC-P IHC-P: Use a concentration of 5 - 10 µg/ml.

Target

  • FunctionRequired for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.
  • Sequence similaritiesContains 3 DRBM (double-stranded RNA-binding) domains.
  • Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Nucleus.

    • Target information above from: UniProt accession Q15633 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      LOQS antibodyPrbp antibodyRISC loading complex subunit TARBP2 antibody
      RISC-loading complex subunit tarbp2 antibodyTAR (HIV 1) RNA binding protein 2 antibodyTAR (HIV) RNA binding protein 2 antibodyTAR (HIV) RNA-binding protein TRBP1 antibodyTAR RNA Binding Protein 2 antibodyTAR RNA-binding protein 2 antibodyTARBP2 antibodyTrans Activation Responsive RNA Binding Protein antibodyTrans-activation-responsive RNA-binding protein antibodyTRBP antibodyTRBP1 antibodyTRBP2 antibodyTRBP2_HUMAN antibody
    see all

Anti-TRBP antibody images

  • Western blot - TRBP antibody (ab72547)
    Western blot - TRBP antibody (ab72547)
    All lanes : Anti-TRBP antibody (ab72547) at 6 µg/ml

    Lane 1 : human brain lysate
    Lane 2 : human brain lysate with immunizing peptide


    Predicted band size : 39 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TRBP antibody (ab72547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TRBP antibody (ab72547)
    ab72547 at 5µg/ml staining TRBP in normal human brain by Immunohistochemistry, Paraffin-embedded tissue.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TRBP antibody (ab72547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TRBP antibody (ab72547)
    Staining of normal human brain by Immunohistochemistry, Paraffin-embedded tissue using a control rabbit Ig antibody at 5µg/ml.
  • Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody (ab72547)
    Immunocytochemistry/ Immunofluorescence - Anti-TRBP antibody (ab72547)This image is courtesy of an anonymous Abreview
    Immunofluorescence analysis of Non-Hodgkin's Lymphoma B cells, staining TRBP with ab72547.

    Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/50 in diluent) for 24 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview

References for Anti-TRBP antibody (ab72547)

ab72547 has not yet been referenced specifically in any publications.

Publishing research using ab72547 ? Please let us know so that we can cite the reference in this datasheet

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-TRBP antibody

 Good
Abreviews
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Adrenocortical carcinoma)
Specification Adrenocortical carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: RT°C
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Verified customer

Submitted Oct 18 2012

I have purchased the ab72547 antibody.
Order no.: 1178970
Invoice no.: 1354346
I have also submitted the Abreview for ab72547 and it has been published on your company website.
Looking forward the validation of my Abtrial discount code XXX...

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Thank you for contacting us.
The Abreview has been published however the image would have also been nice. I have validated the discount code, please order your next antibody by writing the discount code in additional notes box on the order page.
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Immunocytochemistry/ Immunofluorescence abreview for Anti-TRBP antibody

 Good
Abreviews
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (non-Hodgkin B-cell)
Specification non-Hodgkin B-cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X in PBS
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Verified customer

Submitted Oct 08 2012

Thank you for your email.
Yes, I will place a new order for ab72547 (TRBP rabbit polyclonal antibody). Can you send me the 100% Abreview discount code so that I can use it to purchase ab129325.
My next questions are:-
(1) Should I place the n...

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Thank you for contacting us.
Your 100% Abtrial discount code is XXXXXXXX. The expiration date is 07-01-2013. The value of discount code is Euro 370.
Answer to your questions;
- Please place the order either online or by sending email to fax ...

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I am a little confuse about the purchase order number and discount code.
Are you referring to the purchase order number of the previous bought ab72547 antibody? Invoice number: 1255772 (Date 15 Mar, 2011); order reference number: 848489.
Are you a...

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I am sorry for the confusion.
The 100% Abreview discount system works in a way that customers need to get the discount code before the purchase of the product. To validate the code they must buy the product and submitt an Abreview.
It is correct, ...

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I have just submitted the Abreview for ab72547 for ICC/IF application.
Looking forward to hear from you.
Best regards,

Could you please provide me the purchase order number of ab72547? If, the purchase hasn't been done after my offer then I am sorry the offer will not be valid. The discount code offer was for new vial of ab72547 which cannot be used with antibody that is ...

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Dear Sir,
We are interested with your newTRBP monoclonal antibody (ab129325). We have been using theTRBP polyclonal antibody (ab72547) for our cancer research projects (western blot, IF and IHC-P application). We have found interesting results especia...

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Thank you very much for your interest in Abcam products?

Could you please explain why you only need half a vial of each rather than full vials?

We unfortunately cannot provide antibodies in half volume and we can't even provide free samp...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"