TREM1 Human ELISA Kit (ab100659)
- Product nameTREM1 Human ELISA KitSee all TREM1 kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 70 pg/ml
- Range0.069 ng/ml - 50 ng/ml
> 100 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 124.4 106% - 134% Serum 125.5 91% - 145% Plasma 116 94% - 115%
- Species reactivityReacts with: Human
- Product overview
ab100659 Human TREM1 (triggering receptor expressed on myeloid cells 1) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human TREM1 in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human TREM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TREM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TREM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TREM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 500X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent A diluent buffer . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection AntibodyTREM-1 : biotinylated anti-human TREM-1 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml TREM 1 Microplate (12 strips x 8 wells) coated with anti-human TREM-1 1 unit TREM 1 Standard Human(recombinant) 2 vials
- Research Areas
- CD354TREM 1Triggering receptor expressed on monocytes 1
- Triggering receptor expressed on myeloid cells 1Triggering receptor TREM 1Triggering receptor TREM1
Our Abpromise guarantee covers the use of ab100659 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for TREM1 Human ELISA Kit (ab100659)
ab100659 has not yet been referenced specifically in any publications.