Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579)
Key features and details
- Mouse monoclonal [TRF-78] to TRF2 + TRF1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-TRF2 + TRF1 antibody [TRF-78] -
Description
Mouse monoclonal [TRF-78] to TRF2 + TRF1 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human TRF1. Produced in baculovirus.
Database link: P54274 -
Positive control
- ICC/IF: HeLa cells. WB: HeLa, HEK-293, HepG2 and U-2 OS cell lysate. HeLa nuclear cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
TRF-78 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab10579 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 50 kDa.
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ICC/IF | (7) |
1/100.
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Notes |
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WB
Use a concentration of 2 - 4 µg/ml. Predicted molecular weight: 50 kDa. |
ICC/IF
1/100. |
Target
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Cellular localization
TRF2: Nucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase. TRF1: Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle. -
Database links
- Entrez Gene: 7013 Human
- Entrez Gene: 7014 Human
- Omim: 600951 Human
- Omim: 602027 Human
- SwissProt: P54274 Human
- SwissProt: Q15554 Human
- Unigene: 442707 Human
- Unigene: 63335 Human
Images
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All lanes : Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) at 4 µg/ml
Lane 1 : Recombinant Human TRF1 protein cell lysate at 0.1 µg
Lane 2 : Recombinant Human TRF1 protein cell lysate at 0.5 µg
Lane 3 : Empty cell lysate
Lane 4 : Recombinant Human TRF2 protein cell lysate at 0.1 µg
Lane 5 : Recombinant Human TRF2 protein cell lysate at 0.5 µg
Predicted band size: 50 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Anti-TERF antibody [TRF-78] (ab10579) staining at 4 ug/ml, shown in green. In Western blot, ab10579 was shown to bind specifically to TERF. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L 800CW at 1/20000 dilution
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling TRF1 and TRF2 with ab10579. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 10 μg/mL Anti-TRF2 + TRF1 antibody [TRF-78]. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
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All lanes : Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) at 1/1000 dilution
Lane 1 : SAOS2 (Human osteosarcoma cell line) cell lysate
Lane 2 : U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti Mouse polyclonal IRDye 800CW at 1/1000 dilution
Predicted band size: 50 kDa
Exposure time: 5 minutesBlocking stepMilk as blocking agent for 2 hours · Concentration: 5% · Temperature: 21°C.Incubation time12 hours · Temperature: 4°C · Diluent: 5% milk in TBST. -
All lanes : Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) at 4 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 4 : U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate
Lane 5 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
Predicted band size: 50 kDa -
Immunofluorescent imaging of human cells (U2OS) with ab10579 confirms the specificity of this antibody. A few intense nuclear foci are seen in interphase cells, corresponding to telomeric localisation. The complete absence of background nuclear or cytoplasmic staining confirms the specificity of this antibody. This image is in exact agreement with numerous published reports.
IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees. Nuclei are visualised using Hoechst stain.
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ab10579 at 1/500 staining human HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF. The cells were parafomaldehyde fixed and blocked with BSA prior to incubation with the antibody for 45 minutes. An Alexa Fluor® 555 conjugated donkey anti-mouse antibody was used as the secondary.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (66)
ab10579 has been referenced in 66 publications.
- Lee J et al. Dynamic interaction of BRCA2 with telomeric G-quadruplexes underlies telomere replication homeostasis. Nat Commun 13:3396 (2022). PubMed: 35697743
- Barnes RP et al. Telomeric 8-oxo-guanine drives rapid premature senescence in the absence of telomere shortening. Nat Struct Mol Biol 29:639-652 (2022). PubMed: 35773409
- Kychygina A et al. Progerin impairs 3D genome organization and induces fragile telomeres by limiting the dNTP pools. Sci Rep 11:13195 (2021). PubMed: 34162976
- Liu C et al. Paternal USP26 mutations raise Klinefelter syndrome risk in the offspring of mice and humans. EMBO J 40:e106864 (2021). PubMed: 33978233
- Savelyev NV et al. PARP1 Regulates the Biogenesis and Activity of Telomerase Complex Through Modification of H/ACA-Proteins. Front Cell Dev Biol 9:621134 (2021). PubMed: 34095104