Anti-RNF216 antibody (ab226215)
Key features and details
- Rabbit polyclonal to RNF216
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RNF216 antibody
See all RNF216 primary antibodies -
Description
Rabbit polyclonal to RNF216 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Chimpanzee, Rhesus monkey, Orangutan -
Immunogen
Synthetic peptide within Human RNF216 aa 100-150. The exact sequence is proprietary. (NP_996999.1).
Database link: Q9NWF9 -
Positive control
- WB: HeLa, HEK-293T, Jurkat and TCMK-1 whole cell lysates. IP: HeLa whole cell lysate (ab150035).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab226215 was affinity purified using an epitope specific to RNF216 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 99 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 99 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Isoform 1 acts as an E3 ubiquitin ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and then transfers it to substrates promoting their degradation by the proteasome. Promotes degradation of TRAF3, TLR4 and TLR9. Contributes to the regulation of antiviral responses. Down-regulates activation of NF-kappa-B, IRF3 activation and IFNB production. Isoform 3 inhibits TNF and IL-1 mediated activation of NF-kappa-B. Promotes TNF and RIP mediated apoptosis. -
Tissue specificity
Ubiquitous, with the highest levels of expression in testis and peripheral blood leukocytes. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Gordon Holmes syndrome -
Sequence similarities
Contains 1 IBR-type zinc finger.
Contains 2 RING-type zinc fingers. -
Domain
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. -
Post-translational
modificationsAuto-ubiquitinated. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 463248 Chimpanzee
- Entrez Gene: 54476 Human
- Entrez Gene: 108086 Mouse
- Entrez Gene: 100172179 Orangutan
- Entrez Gene: 701819 Rhesus monkey
- Omim: 609948 Human
- SwissProt: Q9NWF9 Human
- SwissProt: P58283 Mouse
see all -
Alternative names
- CAHH antibody
- E3 ubiquitin ligase TRIAD3 antibody
- E3 ubiquitin-protein ligase RNF216 antibody
see all
Images
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All lanes : Anti-RNF216 antibody (ab226215) at 0.4 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 99 kDa
Exposure time: 3 minutes -
RNF216 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226215 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226215 at 1 µg/ml.
Lane 1: ab226215 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226215 has not yet been referenced specifically in any publications.