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Product name
Anti-TRIB3 antibody - Aminoterminal end
See all TRIB3 products (8) ...
Description
Rabbit polyclonal to TRIB3 - Aminoterminal end
Specificity
This antibody is specific to TRIB3. The immunizing sequence has no identity to TRIB1 and TRIB2 isoforms.
Tested applications
WB, IHC-Fr, IPmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Cow
Immunogen
Synthetic peptide: GSLSRKKRLELDDNLDTER, corresponding to N terminal amino acids 11-29 of Human TRIB3 conjugated to KLH
GSLSRKKRLELDDNLDTER
Positive control
HEK-293 cells expressing human TRB3.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: 0.01M PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
ab50516 is affinity purified using the immunizing peptide immobilized on agarose.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cell Biology >> Cell Cycle >> Cell differentiation
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab50516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa.Staining of the TRB3 band is specifically inhibited with the immunizing peptide.
IHC-Fr: 1/200.
IP: Use at an assay dependent dilution.
Target
Function
Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity.
Tissue specificity
Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney.
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
Contains 1 protein kinase domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ96RU7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- C20orf97 antibody
- Neuronal cell death inducible putative kinase antibody
- Neuronal cell death-inducible putative kinase antibody
see all
Database links
- Entrez Gene: 538465 Cow
- Entrez Gene: 57761 Human
- Omim: 607898 Human
- SwissProt: Q0VCE3 Cow
- SwissProt: Q96RU7 Human
- Unigene: 516826 Human
Anti-TRIB3 antibody - Aminoterminal end images:
Western blot - TRIB3 antibody - Aminoterminal end (ab50516)
All lanes : Anti-TRIB3 antibody - Aminoterminal end (ab50516) at 1/1000 dilution
Lane 1 : HEK293 cells transfected with human TRIB3
Lane 2 : HEK293 cells (untransfected)
Lane 3 : HEK293 cells transfected with human TRIB3
with TRIB3 immunizing peptide at 20 µg/ml
Secondary
Goat Anti-Rabbit IgG, Peroxidase conjugate
Predicted band size : 40 kDa
Observed band size : 39 kDa (why is the actual band size different from the predicted?)
Immunohistochemistry (Frozen sections) - TRIB3 antibody - Aminoterminal end (ab50516)
ab50516 staining TRIB3 in human brain tumor tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and permeabilized 0.5% TX-100 before blocking with 10% serum for 1 hour 30 minutes at 250C. The sample was incubated with primary antibody (1/200) in 1% serum in PBS for 2 hours at 250C. An Alexa Fluor®488-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody at 1/500 dilution.
This image is a courtesy of Mar Lorente
References for Anti-TRIB3 antibody - Aminoterminal end (ab50516)
This product has been referenced in:
- Salazar M et al. Cannabinoid action induces autophagy-mediated cell death through stimulation of ER stress in human glioma cells. J Clin Invest 119:1359-72 (2009). IP; Human.Read more (PubMed: 19425170) »
See 1 publication for this product
Publishing research using ab50516? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"