Anti-TRIB3 antibody - Aminoterminal end (ab50516)
Key features and details
- Rabbit polyclonal to TRIB3 - Aminoterminal end
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
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Overview
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Product name
Anti-TRIB3 antibody - Aminoterminal end
See all TRIB3 primary antibodies -
Description
Rabbit polyclonal to TRIB3 - Aminoterminal end -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide:
GSLSRKKRLELDDNLDTER
, corresponding to N terminal amino acids 11-29 of Human TRIB3 conjugated to KLH -
Positive control
- HEK-293 cells expressing human TRB3.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab50516 is affinity purified using the immunizing peptide immobilized on agarose. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa. Staining of the TRB3 band is specifically inhibited with the immunizing peptide.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa. Staining of the TRB3 band is specifically inhibited with the immunizing peptide. |
Target
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Function
Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation. May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1. Binds to ATF4 and inhibits its transcriptional activation activity. Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity. Interacts with MAPK kinases and regulates activation of MAP kinases. May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells. Does not display kinase activity. -
Tissue specificity
Highest expression in liver, pancreas, peripheral blood leukocytes and bone marrow. Also highly expressed in a number of primary lung, colon and breast tumors. Expressed in spleen, thymus, and prostate and is undetectable in other examined tissues, including testis, ovary, small intestine, colon, leukocyte, heart, brain, placenta, lung, skeletal muscle, and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Tribbles subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 57761 Human
- Omim: 607898 Human
- SwissProt: Q96RU7 Human
- Unigene: 516826 Human
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Alternative names
- C20orf97 antibody
- Neuronal cell death inducible putative kinase antibody
- Neuronal cell death-inducible putative kinase antibody
see all
Images
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All lanes : Anti-TRIB3 antibody - Aminoterminal end (ab50516) at 1/1000 dilution
Lane 1 : HEK293 cells transfected with human TRIB3
Lane 2 : HEK293 cells (untransfected)
Lane 3 : HEK293 cells transfected with human TRIB3
with TRIB3 immunizing peptide at 20 µg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate
Predicted band size: 40 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab50516 has been referenced in 5 publications.
- Muñoz-Guardiola P et al. The anti-cancer drug ABTL0812 induces ER stress-mediated cytotoxic autophagy by increasing dihydroceramide levels in cancer cells. Autophagy 17:1349-1366 (2021). PubMed: 32397857
- Felip I et al. Therapeutic potential of the new TRIB3-mediated cell autophagy anticancer drug ABTL0812 in endometrial cancer. Gynecol Oncol 153:425-435 (2019). PubMed: 30853360
- Hu F et al. Knockdown of TRB3 improved the MPP+/MPTP-induced Parkinson's disease through the MAPK and AKT signaling pathways. Neurosci Lett 709:134352 (2019). PubMed: 31283965
- Guan H et al. Competition between members of the tribbles pseudokinase protein family shapes their interactions with mitogen activated protein kinase pathways. Sci Rep 6:32667 (2016). PubMed: 27600771
- Salazar M et al. Cannabinoid action induces autophagy-mediated cell death through stimulation of ER stress in human glioma cells. J Clin Invest 119:1359-72 (2009). IP ; Human . PubMed: 19425170