![Western blot - TRIM5 alpha antibody [3B11H2] (ab31956) image](#ab31956_WB_1.gif)
![Western blot - TRIM5 alpha antibody [3B11H2] (ab31956) image](#ab31956_WB_2.gif)
![Immunohistochemistry (Paraffin-embedded sections) - TRIM5 alpha antibody [3B11H2] (ab31956) image](#ab31956_IHC_1.gif)
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Product name
Anti-TRIM5 alpha antibody [3B11H2]
See all TRIM5 alpha products (6) ...
Description
Mouse monoclonal [3B11H2] to TRIM5 alpha
Tested applications
WB, IHC-P, IHC-Frmore details
Cross reactivity
Reacts with
Human
Immunogen
Ni-NTA purified truncated recombinant human trim5 alpha expressed in E. Coli strain BL21 (DE3).
Positive control
Human breast tissue lysate for Western blot. Human stomach and metastatic adenocarcinoma tissue for Immunohistochemistry.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
3B11H2
Isotype
IgG1
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Ubiquitylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/5000. Predicted molecular weight: 56 kDa.
IHC-P: 1/100 - 1/400.
IHC-Fr
IHC-Fr: 1/200 - 1/400.IHC-Fr: 1/200 - 1/400.
Target
Function
Isoform Alpha is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection. Targets retroviral capsid soon after entry into the cell, and prevents reverse transcription of the virus RNA genome. Isoform Alpha trimers may make multiple contacts with the hexameric lattice of CA proteins which constitute the surface of retrovirion core, and somehow inactivate the virus. Restricts efficiently infection by N-MLV, but not HIV-1. May have E3 ubiquitin-protein ligase activity.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Belongs to the TRIM/RBCC family.
Contains 1 B box-type zinc finger.
Contains 1 B30.2/SPRY domain.
Contains 1 RING-type zinc finger.
Domain
The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme.
Post-translational
modifications
Ubiquitinates itself in a RING finger- and UBE2D2-dependent manner (in vitro).
Cellular localization
Cytoplasm > P-body. Cytoplasmic bodies.
Target information above from: UniProt accessionQ9C035
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RING finger protein 88 antibody
- RNF88 antibody
- TRIM5 antibody
see all
Database links
- Entrez Gene: 85363 Human
- Omim: 608487 Human
- SwissProt: Q9C035 Human
- Unigene: 370515 Human
Anti-TRIM5 alpha antibody [3B11H2] images:
Western blot - TRIM5 alpha antibody [3B11H2] (ab31956)
![Western blot - TRIM5 alpha antibody [3B11H2] (ab31956)](/ps/datasheet/Images/31/ab31956/ab31956_WB_1.gif)
All lanes : Anti-TRIM5 alpha antibody [3B11H2] (ab31956)
Lane 1 : Molecular weight marker
Lane 2 : TRIM5-alpha recombinant protein (NB: truncated form)
Predicted band size : 56 kDa
Observed band size : ~30 kDa (why is the actual band size different from the predicted?)
Western blot - TRIM5 alpha antibody [3B11H2] (ab31956)
![Western blot - TRIM5 alpha antibody [3B11H2] (ab31956)](/ps/datasheet/Images/31/ab31956/ab31956_WB_2.gif)
All lanes : Anti-TRIM5 alpha antibody [3B11H2] (ab31956)
Lane 1 : Molecular weight marker
Lane 2 : Breast tissue lysate
Predicted band size : 56 kDa
Observed band size : ~57 kDa (why is the actual band size different from the predicted?)
Additional bands at : ~30 kDa (possible truncated form).
Blot imaged using ECL (Pierce's SuperSignal Femto substrate)
Immunohistochemistry (Paraffin-embedded sections) - TRIM5 alpha antibody [3B11H2] (ab31956)
![Immunohistochemistry (Paraffin-embedded sections) - TRIM5 alpha antibody [3B11H2] (ab31956)](/ps/datasheet/Images/31/ab31956/ab31956_IHC_1.gif)
Immunohistochemical analysis of paraffin-embedded human metastatic adenocarcinoma, showing cytoplasmic localisation, using anti-human TRIM5 alpha antibody (ab31956). A secondary HRP-conjugated antibody was used before colour development with AEC.
Immunohistochemistry (Paraffin-embedded sections) - TRIM5 alpha antibody [3B11H2] (ab31956)
![Immunohistochemistry (Paraffin-embedded sections) - TRIM5 alpha antibody [3B11H2] (ab31956)](/ps/datasheet/Images/31/ab31956/ab31956_IHC_2.gif)
Immunohistochemical analysis of human stomach adenocarcinoma, showing cytoplasmic localisation, using anti-human TRIM5 alpha antibody (ab31956). A secondary HRP-conjugated antibody was used before colour development with DAB.
References for Anti-TRIM5 alpha antibody [3B11H2] (ab31956)
ab31956 has not yet been referenced specifically in any publications.
Publishing research using ab31956? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"