Anti-GMAP-210 antibody (ab72223)
Key features and details
- Rabbit polyclonal to GMAP-210
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-GMAP-210 antibody
See all GMAP-210 primary antibodies -
Description
Rabbit polyclonal to GMAP-210 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human GMAP-210 aa 1-100.
Database link: Q15643 -
Positive control
- Whole cell lysates from HeLa and 293T cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab72223 was affinity purified using an epitope specific to GMAP-210 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 238 kDa (predicted molecular weight: 228 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 238 kDa (predicted molecular weight: 228 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Relevance
TRIP230 was identified through its ability to interact functionally with thyroid hormone receptor-beta. It has also been found in association with the Golgi apparatus and microtubules. TRIP230 binds the ligand binding domain of the thyroid receptor (THRB) in the presence of triiodothyronine and enhances THRB-modulated transcription. This protein is probably involved in maintaining cis-Golgi structure. -
Cellular localization
Cell Membrane and Golgi Apparatus -
Database links
- Entrez Gene: 9321 Human
- Omim: 604505 Human
- SwissProt: Q15643 Human
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Alternative names
- CEV 14 antibody
- CEV14 antibody
- Clonal evolution related gene on chromosome 14 antibody
see all
Images
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All lanes : Anti-GMAP-210 antibody (ab72223) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 228 kDa
Observed band size: 238 kDa why is the actual band size different from the predicted?
Exposure time: 3 seconds -
Detection of Human GMAP-210 by Immunoprecipitation. 1mg HeLa whole cell lysate loaded in lanes 1-3 with ab72223 at 3µg in lane 1, an antibody recognising a downstream epitope in lane 2 and control IgG in lane 3. Image shows immunoprecipitated GMAP-210 detected with post IP WB, loading 20% of IP and using ab72223 at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab72223 has been referenced in 1 publication.
- Snyder JC et al. Constitutive internalization of the leucine-rich G protein-coupled receptor-5 (LGR5) to the trans-Golgi network. J Biol Chem 288:10286-97 (2013). PubMed: 23439653