Anti-TRP 7 antibody [N64A/36] (ab93618)
Key features and details
- Mouse monoclonal [N64A/36] to TRP 7
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-TRP 7 antibody [N64A/36] -
Description
Mouse monoclonal [N64A/36] to TRP 7 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide:
LRVNKGKDI
, corresponding to amino acids 854-862 of Human TRP 7 (UniProt Q9HCX4). -
Positive control
- Rat brain lysate
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General notes
The clone number has been updated from S64A-36 to N64A/36, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N64A/36 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab93618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
IHC-P | ||
ICC/IF |
Notes |
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ICC/IF: Use at a concentration of 1 - 10 µg/ml.
IHC-P: Use at a concentration of 0.1 - 1 µg/ml.
IHC-Fr: Use at a concentration of 0.1 - 1 µg/ml.
WB: Use at a concentration of 1 - 10 µg/ml. Predicted molecular weight: 100 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) (By similarity). May also be activated by intracellular calcium store depletion. -
Sequence similarities
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC7 sub-subfamily.
Contains 4 ANK repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 57113 Human
- Entrez Gene: 26946 Mouse
- Entrez Gene: 282822 Rat
- SwissProt: Q9HCX4 Human
- SwissProt: Q9WVC5 Mouse
- Unigene: 591263 Human
- Unigene: 62409 Mouse
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Alternative names
- Capacitative calcium channel TRPC7 antibody
- cation channel, subfamily C, member 7 antibody
- hTRP7 antibody
see all
Images
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Western blot detection of Trp7 in Rat brain membrane lysates using ab93618 at 1:1000 dilution.
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Cold 100% methanol for 10 minutes at -20°C fixed HaCaT cells labeling TRP 7 (green) using ab93618 at 1/100 dilution for 1 hour at room temperature. FITC Goat anti-mouse (green) was used as the secondary antibody at 1/50 dilution for 1 hour at room temperature. Nuclear staining is observed.
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Bouin's-fixed, paraffin-embedded mouse backskin tissue stained for TRP 7 (green) using ab93618 at 1/100 dilution in immunohistochemical analysis. Goat Anti-Mouse was used as the secodary antibody at 1/50 dilution.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab93618 has been referenced in 1 publication.
- Nakipova OV et al. Store-operated Ca2+ entry supports contractile function in hearts of hibernators. PLoS One 12:e0177469 (2017). PubMed: 28531217