Anti-TRP2/DCT antibody (ab74073)
Key features and details
- Rabbit polyclonal to TRP2/DCT
- Suitable for: ELISA, IHC-P, IHC-Fr, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TRP2/DCT antibody
See all TRP2/DCT primary antibodies -
Description
Rabbit polyclonal to TRP2/DCT -
Host species
Rabbit -
Tested applications
Suitable for: ELISA, IHC-P, IHC-Fr, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human TRP2/DCT (internal sequence).
Database link: P40126 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab74073 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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IHC-P | (2) |
Use at an assay dependent concentration.
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IHC-Fr | (2) |
Use at an assay dependent concentration.
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WB | (2) |
1/500 - 1/1000. Predicted molecular weight: 59 kDa.
TRP2 protein is a single-pass type I membrane protein, these types of proteins are known to aggregate when boiled, therefore, we recommend heating the lysates in sample buffer for 15-20 minutes at 60-70°C instead of boiling. |
ICC/IF | (2) |
1/100 - 1/500.
|
Notes |
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ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-Fr
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Predicted molecular weight: 59 kDa. TRP2 protein is a single-pass type I membrane protein, these types of proteins are known to aggregate when boiled, therefore, we recommend heating the lysates in sample buffer for 15-20 minutes at 60-70°C instead of boiling. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Involved in regulating eumelanin and phaeomelanin levels. -
Pathway
Pigment biosynthesis; melanin biosynthesis. -
Sequence similarities
Belongs to the tyrosinase family. -
Cellular localization
Melanosome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1638 Human
- Entrez Gene: 13190 Mouse
- Omim: 191275 Human
- SwissProt: P40126 Human
- SwissProt: P29812 Mouse
- Unigene: 301865 Human
- Unigene: 19987 Mouse
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Alternative names
- L dopachrome tautomerase antibody
- DCT antibody
- Dopachrome delta isomerase antibody
see all
Images
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All lanes : Anti-TRP2/DCT antibody (ab74073) at 1/500 dilution
Lane 1 : Extracts from K562 cells with no peptide
Lane 2 : Extracts from K562 cells with peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 59 kDa
Observed band size: 59 kDa
Additional bands at: 117 kDa, 39 kDa. We are unsure as to the identity of these extra bands. -
Immunofluorescence analysis of TRP2/DCT in A549 cells, using ab74073 at a dilution of 1/500.
Left hand panel: no peptide
right hand panel: with immunising peptide. -
ab74073 staining TRP2/DCT in Mouse melan-ink melanocytes by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 30 hours at room temperature. Samples were incubated with primary antibody (1/100 in PBS -T+ 1% BSA) for 12 hours. An undiluted Alexa Fluor® 488-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (51)
ab74073 has been referenced in 51 publications.
- Wohlfeil SA et al. Angiogenic and molecular diversity determine hepatic melanoma metastasis and response to anti-angiogenic treatment. J Transl Med 20:62 (2022). PubMed: 35109875
- Hu SH et al. sPmel17 Secreted by Ultraviolet B-Exposed Melanocytes Alters the Intercellular Adhesion of Keratinocytes. Oxid Med Cell Longev 2022:1856830 (2022). PubMed: 35186181
- Tanwar J et al. Mitofusin-2 Negatively Regulates Melanogenesis by Modulating Mitochondrial ROS Generation. Cells 11:N/A (2022). PubMed: 35203350
- Bonnamour G et al. NR2F1 regulates a Schwann cell precursor-vs-melanocyte cell fate switch in a mouse model of Waardenburg syndrome type IV. Pigment Cell Melanoma Res 35:506-516 (2022). PubMed: 35816394
- Morante V et al. Integrase-Defective Lentiviral Vector Is an Efficient Vaccine Platform for Cancer Immunotherapy. Viruses 13:N/A (2021). PubMed: 33672349