• Product name
  • Description
    Rabbit polyclonal to TRPC6
  • Host species
  • Tested applications
    Suitable for: IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide corresponding to Mouse TRPC6 aa 24-38 (N terminal).


    Database link: Q61143

  • Positive control
    • Rat brain.
  • General notes
    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.



Our Abpromise guarantee covers the use of ab12249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration.
WB 1/200 - 1/400. Predicted molecular weight: 111 kDa.


  • Function
    Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C. Seems not to be activated by intracellular calcium store depletion.
  • Tissue specificity
    Expressed primarily in placenta, lung, spleen, ovary and small intestine. Expressed in podocytes and is a component of the glomerular slit diaphragm.
  • Involvement in disease
    Defects in TRPC6 are the cause of focal segmental glomerulosclerosis type 2 (FSGS2) [MIM:603965]. A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation.
  • Sequence similarities
    Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC6 sub-subfamily.
    Contains 4 ANK repeats.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • bZ1P14.9 antibody
    • FLJ11098 antibody
    • FLJ14863 antibody
    • FSGS2 antibody
    • MTRP6 antibody
    • Short transient receptor potential channel 6 antibody
    • si:rp71-1p14.9 antibody
    • Transient receptor potential cation channel subfamily C member 6 antibody
    • Transient receptor protein 6 antibody
    • TRP 6 antibody
    • TRP-6 antibody
    • TRP6 antibody
    • TRPC 6 antibody
    • Trpc6 antibody
    • TRPC6_HUMAN antibody
    • TRRP6 antibody
    see all


  • Lane 1:Anti-TRPC6 antibody, (1:200).

    Lane 2: Anti-TRPC6 antibody, preincubated with the control peptide antigen. 

  • ab12249 staining TRPC6 in mouse Olfactory tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections). Mice were anesthetized with 20% chloral hydrate (2 mg/g body weight), perfused transcardially with 0.9% saline followed by 4% paraformaldehyde in 0.1 M phosphate buffer (PB). The olfactory organs were dissected and postfixed in the same fixative for 15 min to overnight. Cryosections were rinsed in 0.1 M phosphate buffered saline (PBS), blocked in blocking solution containing 1% BSA, 3% normal donkey serum, and 0.3% Triton X-100 in PBS for 2 hours, and then incubated in the primary antisera overnight to 2 days. An Alexa Fluor® 568 conjugated anti rabbit IgG was used for 2 hours as secondary. ab12249 stains red in image shown.


This product has been referenced in:
  • Letavernier E  et al. Williams-Beuren syndrome hypercalcemia: is TRPC3 a novel mediator in calcium homeostasis? Pediatrics 129:e1626-30 (2012). ICC/IF, IHC ; Human . Read more (PubMed: 22566418) »
  • Li W  et al. Activity-dependent BDNF release and TRPC signaling is impaired in hippocampal neurons of Mecp2 mutant mice. Proc Natl Acad Sci U S A 109:17087-92 (2012). WB ; Mouse . Read more (PubMed: 23027959) »

See all 5 Publications for this product

Customer reviews and Q&As

Thank you for your email. With ab12249 you should see a band at approximately 111 kDa. It sounds that perhaps there is an unreported novel protein that shares a similar epitope and could possibly be from the same protein family that is being detected. ...

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Thank you for your email. The free of charge replacement vial is being sent to your attention on order reference # 109650. The item is currently out of stock and you should receive the replacement early next week. You will receive emails regarding ship...

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Thank you for the details that you have provided and I'm sorry to hear that you are experiencing difficulty with ab12249. This antibody should recognize the TRPC6 protein from rat brain and I do not see any problems with your protocol. I therefore can ...

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Thank you for your enquiry. I would suggest using rat brain as a positive control for this antibody. If you have any additional questions, please contact us again.


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