Anti-TRPM8 antibody (ab71245)

Overview

  • Product nameAnti-TRPM8 antibodySee all TRPM8 primary antibodies ...
  • Description
    Rabbit polyclonal to TRPM8
  • Tested applicationsWB, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A KLH conjugated synthetic peptide at the center(R536) of human TRPM8.

  • Positive control
    • Transfected/ non-transfected 293 cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab71245 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/50 - 1/100. Detects a band of approximately 57, 128 kDa (predicted molecular weight: 128 kDa).
IHC-P 1/10 - 1/50.
ELISA 1/1000.

Target

  • FunctionReceptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius. Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium. Temperature sensing is tightly linked to voltage-dependent gating. Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves. The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials. Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
  • Tissue specificityExpressed in prostate. Also expressed in most in prostate tumors. Also expressed in non-prostatic primary tumors such as colon, lung, breast and skin tumors.
  • Sequence similaritiesBelongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM8 sub-subfamily.
  • Cellular localizationMembrane.
  • Target information above from: UniProt accession Q7Z2W7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Long transient receptor potential channel 6 antibody
    • LTrpC-6 antibody
    • LTrpC6 antibody
    • MGC2849 antibody
    • Short form of the TRPM8 cationic channel antibody
    • Transient receptor potential cation channel subfamily M member 8 antibody
    • Transient receptor potential p8 antibody
    • transient receptor potential-p8 antibody
    • Trp p8 antibody
    • Trp-p8 antibody
    • Trpm8 antibody
    • TRPM8_HUMAN antibody
    • TRPP8 antibody
    see all

Anti-TRPM8 antibody images

  • All lanes : Anti-TRPM8 antibody (ab71245) at 1/50 dilution

    Lane 1 : Non-transfected 293 cell lysates
    Lane 2 : TRPM8 transfected 293 cell lysates

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 128 kDa
    Observed band size : 128 kDa
    Additional bands at : 57 kDa. We are unsure as to the identity of these extra bands.
  • ab71245 at 1/50 dilution staining TRPM8 in human prostate carcinoma tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References for Anti-TRPM8 antibody (ab71245)

ab71245 has not yet been referenced specifically in any publications.

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Thank you for your interest in these antibodies. Without a discount the prices range from €360.00 to €370.00. A bulk order discount of approximately 5% is available for orders of 5 units or more of 5 different antibodies. The discount my collea...

Read More
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Dental pulp)
Specification Dental pulp
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Andrew Grant

Verified customer

Submitted Mar 28 2011

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Tongue)
Specification Tongue
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization No
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Dr. Andrew Grant

Verified customer

Submitted Dec 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"