Anti-TRPV3 antibody [N15/4] (ab85022)
Key features and details
- Mouse monoclonal [N15/4] to TRPV3
- Suitable for: WB, Flow Cyt, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-TRPV3 antibody [N15/4]
See all TRPV3 primary antibodies -
Description
Mouse monoclonal [N15/4] to TRPV3 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide corresponding to Rat TRPV3 aa 774-791. From the cytoplasmic C-terminus
Sequence:KTTLYAFDELDEFPETSV
Database link: NP_001020928 -
Positive control
- COS-1 cell lysate transiently transfected with TRPV3.
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General notes
The clone number has been updated from S15-4 to N15/4, both clone numbers name the same antibody clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
N15/4 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa.
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Flow Cyt |
Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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IHC-P | (2) |
1/1000.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 90 kDa. |
Flow Cyt
Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
IHC-P
1/1000. |
Target
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Function
Putative receptor-activated non-selective calcium permeant cation channel. It is activated by innocuous (warm) temperatures and shows an increased response at noxious temperatures greater than 39 degrees Celsius. Activation exhibits an outward rectification. May associate with TRPV1 and may modulate its activity. -
Tissue specificity
Abundantly expressed in CNS. Widely expressed at low levels. Detected in dorsal root ganglion (at protein level). -
Sequence similarities
Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV3 sub-subfamily.
Contains 3 ANK repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 523538 Cow
- Entrez Gene: 162514 Human
- Entrez Gene: 246788 Mouse
- Omim: 607066 Human
- SwissProt: Q8NET8 Human
- SwissProt: Q8K424 Mouse
- Unigene: 446255 Human
- Unigene: 347652 Mouse
see all -
Alternative names
- 1110036I10Rik antibody
- MGC124324 antibody
- MGC124325 antibody
see all
Images
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Immunohistochemical analysis of Bouin's Fixative and paraffin-embedded Mouse backskin tissue labelling TrpV3 with ab85022 at 1/100 dilution (1 hour at RT), followed by FITC Goat Anti-Mouse (green) at 1/50 (1 hour at RT).
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Overlay histogram showing SH-SY5Y cells stained with ab85022 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85022, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
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All lanes : Anti-TRPV3 antibody [N15/4] (ab85022) at 1 µg/ml
Lane 1 : Molecular weight marker
Lane 2 : Cell lysates prepared from mixture of human cell lines A431, A549, HCT116, HeLa, HEK293, HepG2, HL-60, HUVEC, Jurkat, MCF7, PC3 and T98G at 15 µg
Secondary
Lane 2 : Sheep Anti-Mouse IgG: HRP for 1 hour at RT
Predicted band size: 90 kDaBlock: 1.5% BSA for 30 minutes at RT
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Immunohistochemical analysis of Bouin's Fixative and paraffin-embedded Human hippocampus tissue labelling TrpV3 with ab85022 at 1/1000 dilution (1 hour at RT), followed by FITC Goat Anti-Mouse (green) at 1/50 (1 hour at RT).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab85022 has been referenced in 7 publications.
- Jiang Y et al. Multi-Omics Analysis of the Prognosis and Biological Function for TRPV Channel Family in Clear Cell Renal Cell Carcinoma. Front Immunol 13:872170 (2022). PubMed: 35558077
- Foote AG et al. Localization of TRPV3/4 and PIEZO1/2 sensory receptors in murine and human larynges. Laryngoscope Investig Otolaryngol 7:1963-1972 (2022). PubMed: 36544955
- Keshri GK et al. Effects of Pulsed 810?nm Al-Ga-As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight. Lasers Surg Med 52:424-436 (2020). PubMed: 31483061
- Seo SH et al. Enhanced Thermal Sensitivity of TRPV3 in Keratinocytes Underlies Heat-Induced Pruritogen Release and Pruritus in Atopic Dermatitis. J Invest Dermatol 140:2199-2209.e6 (2020). PubMed: 32247859
- Sakakibara A et al. Upregulated Expression of Transient Receptor Potential Cation Channel Subfamily V Receptors in Mucosae of Patients with Oral Squamous Cell Carcinoma and Patients with a History of Alcohol Consumption or Smoking. PLoS One 12:e0169723 (2017). PubMed: 28081185
- Pires PW et al. Unitary TRPV3 channel Ca2+ influx events elicit endothelium-dependent dilation of cerebral parenchymal arterioles. Am J Physiol Heart Circ Physiol 309:H2031-41 (2015). PubMed: 26453324
- Albert ES et al. TRPV4 channels mediate the infrared laser-evoked response in sensory neurons. J Neurophysiol 107:3227-34 (2012). PubMed: 22442563