Anti-TSG101 antibody (ab70974)

Overview

• Product nameAnti-TSG101 antibodySee all TSG101 primary antibodies ...
• Description
  Rabbit polyclonal to TSG101
• Tested applicationsWB, ELISA more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide from the C-terminal region of human TSG101, conjugated to KLH.

• Positive controlZR75-1 cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • Other

  • Cancer
  • Cell cycle
  • Cell differentiation

  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • Other

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Differentiation

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Other

Applications

Target

• FunctionComponent of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses.
• Tissue specificityHeart, brain, placenta, lung, liver, skeletal, kidney and pancreas.
• Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family. UEV subfamily.
  Contains 1 SB (steadiness box) domain.
  Contains 1 UEV (ubiquitin E2 variant) domain.
• DomainThe UEV domain is required for the interaction of the complex with ubiquitin. It also mediates the interaction with PTAP/PSAP motifs of HIV-1 P6 protein and human spumaretrovirus Gag protein.
  The coiled coil domain may interact with stathmin.
  The UEV domain binds ubiquitin and P-[ST]-A-P peptide motif independently.
• Post-translational
  modificationsMonoubiquitinated at multiple sites by LRSAM1 and by MGRN1. Ubiquitination inactivates it, possibly by regulating its shuttling between an active membrane-bound protein and an inactive soluble form. Ubiquitination by MGRN1 requires the presence of UBE2D1.
• Cellular localizationCytoplasm. Membrane. Nucleus. Late endosome membrane. Mainly cytoplasmic. Membrane-associated when active and soluble when inactive. Depending on the stage of the cell cycle, detected in the nucleus. Colocalized with CEP55 in the midbody during cytokinesis.

Target information above from: UniProt accession Q99816 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7251 Human
  • Omim: 601387 Human
  • SwissProt: Q99816 Human
  • Unigene: 523512 Human

• Alternative names
  ESCRT I complex subunit TSG101 antibodyESCRT-I complex subunit TSG101 antibodyTS101_HUMAN antibody

  TSG 10 antibodyTSG 101 antibodyTSG10 antibodyTsg101 antibodyTumor susceptibility gene 10 antibodyTumor susceptibility gene 101 antibodyTumor susceptibility gene 101 protein antibodyTumor susceptibility protein antibodyTumor susceptibility protein isoform 3 antibodyVPS 23 antibodyVPS23 antibody

  see all

Anti-TSG101 antibody images

• 

  Western blot - TSG101 antibody (ab70974)
  Anti-TSG101 antibody (ab70974) at 1/100 dilution + ZR75-1 cell lysate at 12.5 µg
  
  Predicted band size : 44 kDa
  Observed band size : 44 kDa