Overview

  • Product nameAnti-TUBA1A antibody
    See all TUBA1A primary antibodies
  • Description
    Mouse monoclonal to TUBA1A
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: KVGINYQPPT VVPGGDLAKV QRAVCMLSNT TAIAEAWARL DHKFDLMYAK RAFVHWYVGE GMEEGEFSEA REDMAALEKD YEEVGVDSVE GEGEEEGEEY , corresponding to amino acids 352-452 of Human TUBA1A, with tag

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Monoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab76449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.
ELISA Use at an assay dependent dilution. Detection limit for ab76449 is approximately 0.3ng/ml when used as a capture antibody.

Target

  • RelevanceTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Cellular localizationmicrotubule
  • Database links
  • Alternative names
    • Alpha tubulin 3 antibody
    • B alpha 1 antibody
    • FLJ25113 antibody
    • LIS3 antibody
    • TUBA3 antibody
    • Tubulin alpha 1a antibody
    • Tubulin alpha 1A chain antibody
    • Tubulin alpha 3 antibody
    • Tubulin alpha 3 chain antibody
    • Tubulin alpha brain specific antibody
    • Tubulin B alpha 1 antibody
    see all

Anti-TUBA1A antibody images

  • Anti-TUBA1A antibody (ab76449) at 1/500 dilution + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 50 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-TUBA1A antibody (ab76449) at 1/500 dilution + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

References for Anti-TUBA1A antibody (ab76449)

ab76449 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"