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Overview

  • Product nameAnti-TUBA1A antibodySee all TUBA1A primary antibodies ...
  • Description
    Mouse monoclonal to TUBA1A
  • Tested applicationsWB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: KVGINYQPPT VVPGGDLAKV QRAVCMLSNT TAIAEAWARL DHKFDLMYAK RAFVHWYVGE GMEEGEFSEA REDMAALEKD YEEVGVDSVE GEGEEEGEEY , corresponding to amino acids 352-452 of Human TUBA1A, with tag

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Monoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab76449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Predicted molecular weight: 50 kDa.
ELISA ELISA: Use at an assay dependent dilution. Detection limit for ab76449 is approximately 0.3ng/ml when used as a capture antibody.

Target

  • Alternative names
      Alpha tubulin 3 antibodyB alpha 1 antibodyFLJ25113 antibody
      LIS3 antibodyTUBA3 antibodyTubulin alpha 1a antibodyTubulin alpha 1A chain antibodyTubulin alpha 3 antibodyTubulin alpha 3 chain antibodyTubulin alpha brain specific antibodyTubulin B alpha 1 antibody
    see all

Anti-TUBA1A antibody images

  • Western blot - TUBA1A antibody [2D2] (ab76449)
    Western blot - TUBA1A antibody [2D2] (ab76449)
    Anti-TUBA1A antibody (ab76449) at 1/500 dilution + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 50 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Western blot - TUBA1A antibody [2D2] (ab76449)
    Western blot - TUBA1A antibody [2D2] (ab76449)
    Anti-TUBA1A antibody (ab76449) at 1/500 dilution + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

References for Anti-TUBA1A antibody (ab76449)

ab76449 has not yet been referenced specifically in any publications.

Publishing research using ab76449 ? Please let us know so that we can cite the reference in this datasheet

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"