Directly conjugated antibodies for immunofluorescence

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Overview

  • Product nameAnti-TUBA1A antibody
    See all TUBA1A primary antibodies
  • Description
    Mouse monoclonal to TUBA1A
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment: KVGINYQPPT VVPGGDLAKV QRAVCMLSNT TAIAEAWARL DHKFDLMYAK RAFVHWYVGE GMEEGEFSEA REDMAALEKD YEEVGVDSVE GEGEEEGEEY , corresponding to amino acids 352-452 of Human TUBA1A, with tag

  • Positive control
    • HepG2 cell lysate

Properties

  • FormLiquid
  • Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • Clonality Monoclonal
  • IsotypeIgG2a
  • Light chain typekappa
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab76449 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.
ELISA Use at an assay dependent dilution. Detection limit for ab76449 is approximately 0.3ng/ml when used as a capture antibody.

Target

  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Tissue specificityExpressed at a high level in fetal brain.
  • Involvement in diseaseLissencephaly 3
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    modifications
    Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localizationCytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha tubulin 3 antibody
    • Alpha-tubulin 3 antibody
    • B alpha 1 antibody
    • FLJ25113 antibody
    • LIS3 antibody
    • TBA1A_HUMAN antibody
    • TUBA1A antibody
    • TUBA3 antibody
    • Tubulin alpha 1a antibody
    • Tubulin alpha 1A chain antibody
    • Tubulin alpha 3 antibody
    • Tubulin alpha 3 chain antibody
    • Tubulin alpha brain specific antibody
    • Tubulin alpha-1A chain antibody
    • Tubulin alpha-3 chain antibody
    • Tubulin B alpha 1 antibody
    • Tubulin B-alpha-1 antibody
    see all

Anti-TUBA1A antibody images

  • Anti-TUBA1A antibody (ab76449) at 1/500 dilution + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 50 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
  • Anti-TUBA1A antibody (ab76449) at 1/500 dilution + HepG2 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 50 kDa
    Observed band size : 50 kDa

References for Anti-TUBA1A antibody (ab76449)

ab76449 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"