• Product nameAnti-TUBA1A antibody
    See all TUBA1A primary antibodies
  • Description
    Rabbit polyclonal to TUBA1A
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 37-286 of Human TUBA1A (NP_006000).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lysate. A431 cells


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas


Our Abpromise guarantee covers the use of ab95966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 50 kDa.
ICC/IF 1/100 - 1/200.


  • FunctionTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
  • Tissue specificityExpressed at a high level in fetal brain.
  • Involvement in diseaseLissencephaly 3
  • Sequence similaritiesBelongs to the tubulin family.
  • Post-translational
    Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively.
    Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
    Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
  • Cellular localizationCytoplasm, cytoskeleton.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha tubulin 3 antibody
    • Alpha-tubulin 3 antibody
    • B alpha 1 antibody
    • FLJ25113 antibody
    • LIS3 antibody
    • TBA1A_HUMAN antibody
    • TUBA1A antibody
    • TUBA3 antibody
    • Tubulin alpha 1a antibody
    • Tubulin alpha 1A chain antibody
    • Tubulin alpha 3 antibody
    • Tubulin alpha 3 chain antibody
    • Tubulin alpha brain specific antibody
    • Tubulin alpha-1A chain antibody
    • Tubulin alpha-3 chain antibody
    • Tubulin B alpha 1 antibody
    • Tubulin B-alpha-1 antibody
    see all

Anti-TUBA1A antibody images

  • All lanes : Anti-TUBA1A antibody (ab95966) at 1/10000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate
    Lane 3 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 50 kDa
  • ab95966 at 1/200 dilution staining TUBA1A in paraformaldehyde-fixed A431 cells by immunofluorescence. ab95966 has been merged with a DNA probe in the lower image.

References for Anti-TUBA1A antibody (ab95966)

ab95966 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab95966.
Please use the links above to contact us or submit feedback about this product.