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Overview

  • Product nameTUBA1A protein (Tagged-His Tag)See all TUBA1A proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 163-451 (289 amino acids) of Human TUBA1A with N terminal His tag; Predicted MWt 33 kDa (UniProt Q71U36).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 111 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch)
  • Sequence notesKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAF MVDNEAIY ICRRNLDIERPTYTNLNRLIGQIVSSITAS LRFDGALNVDLTEFQTNL VPYPRIHFPLATYAPVISAE KAYHEQLSVAEITNACFEPANQMVKCDP RHGKYMACCL LYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGI NYQPPTVVPGGDLAKVQRAVCMLSNTTAIAEAWARLDHKF DLMYAKRA FVHWYVGEGMEEGEFSEAREDMAALEKDYE EVGVDSVEGEGEEEGEEY
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92108 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Alpha tubulin 3B alpha 1FLJ25113
        LIS3TUBA3Tubulin alpha 1aTubulin alpha 1A chainTubulin alpha 3Tubulin alpha 3 chainTubulin alpha brain specificTubulin B alpha 1
      see all
  • RelevanceTubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
  • Cellular localizationmicrotubule

    • TUBA1A protein (Tagged-His Tag) images

    • SDS-PAGE - TUBA1A protein (Tagged-His Tag) (ab92108)
      SDS-PAGE - TUBA1A protein (Tagged-His Tag) (ab92108)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for TUBA1A protein (Tagged-His Tag) (ab92108)

    ab92108 has not yet been referenced specifically in any publications.

    Publishing research using ab92108 ? Please let us know so that we can cite the reference in this datasheet

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