The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Peptide ELISA: Antibody detection limit dilution 1/32000.
WB: Use at a concentration of 0.2 - 0.6 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 47 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Mediates thiol-dependent retention in the early secretory pathway, forming mixed disulfides with substrate proteins through its conserved CRFS motif. Inhibits the calcium channel activity of ITPR1. May have a role in the control of oxidative protein folding in the endoplasmic reticulum. Required to retain ERO1L and ERO1LB in the endoplasmic reticulum.
Anti-TXNDC4 antibody (ab77403) at 0.2 µg/ml + K562 lysate protein in RIPA buffer at 35 µg
Predicted band size: 47 kDa Observed band size: 47 kDa Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.
Primary incubation was 1 hour. Detected by chemiluminescence. An additional band of unknown identity was consistently observed at 22kDa. This band was successfully blocked by incubation with the immunising peptide.