Anti-TXNDC5 antibody (ab13820)
Key features and details
- Goat polyclonal to TXNDC5
- Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-TXNDC5 antibody
See all TXNDC5 primary antibodies -
Description
Goat polyclonal to TXNDC5 -
Host species
Goat -
Specificity
This antibody is expected to be able to recognise both reported human isoforms, as represented by NP_110437; NP_071368. -
Tested applications
Suitable for: IHC-P, WB, Flow Cyt (Intra), ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human TXNDC5 aa 400 to the C-terminus (C terminal). (NP_001139021.1)
Database link: NP_110437.2 -
Positive control
- Flow Cyt (Intra): HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13820 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
Use at an assay dependent concentration.
|
|
WB |
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
A 1 hour primary incubation is recommended for this product. |
|
Flow Cyt (Intra) |
Use a concentration of 10 µg/ml.
|
|
ICC/IF | (1) |
Use a concentration of 10 µg/ml.
|
Notes |
---|
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). A 1 hour primary incubation is recommended for this product. |
Flow Cyt (Intra)
Use a concentration of 10 µg/ml. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
-
Function
Possesses thioredoxin activity. Has been shown to reduce insulin disulfide bonds. Also complements protein disulfide-isomerase deficiency in yeast. -
Sequence similarities
Belongs to the protein disulfide isomerase family.
Contains 3 thioredoxin domains. -
Cellular localization
Endoplasmic reticulum lumen. - Information by UniProt
-
Database links
- Entrez Gene: 81567 Human
- SwissProt: Q8NBS9 Human
- Unigene: 150837 Human
-
Alternative names
- EndoPDI antibody
- Endoplasmic reticulum protein ERp46 antibody
- Endoplasmic reticulum resident protein 46 antibody
see all
Images
-
All lanes : Anti-TXNDC5 antibody (ab13820) at 0.1 µg/ml
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : TXNDC5 knockout HEK-293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : A549 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-TXNDC5 antibody staining at 0.1 µg/ml, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab13820 was shown to bind specifically to TXNDC5. A band was observed at 47 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in TXNDC5 knockout cell line ab266609 (knockout cell lysate ab263403). To generate this image, wild-type and TXNDC5 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216778) at 1/20000 dilution.
-
Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation with ab13820 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
-
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation with ab13820 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (1 μg/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
-
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab13820 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing endoplasmic reticulum staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
-
All lanes : Anti-TXNDC5 antibody (ab13820) at 0.1 µg/ml
Lane 1 : HEK-293 cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 50 kDa
Protocols
Datasheets and documents
-
Datasheet download
References (6)
ab13820 has been referenced in 6 publications.
- Chen X et al. Inhibition of ER stress by targeting the IRE1a-TXNDC5 pathway alleviates crystalline silica-induced pulmonary fibrosis. Int Immunopharmacol 95:107519 (2021). PubMed: 33691254
- Zang H et al. Circ_0000517 Contributes to Hepatocellular Carcinoma Progression by Upregulating TXNDC5 via Sponging miR-1296-5p. Cancer Manag Res 12:3457-3468 (2020). PubMed: 32523376
- Wang L et al. miR-573 is a negative regulator in the pathogenesis of rheumatoid arthritis. Cell Mol Immunol 13:839-849 (2016). PubMed: 26166764
- Wang L et al. Investigate pathogenic mechanism of TXNDC5 in rheumatoid arthritis. PLoS One 8:e53301 (2013). WB, IHC-P ; Mouse . PubMed: 23326410
- Pace PE et al. Hyperoxidized peroxiredoxin 2 interacts with the protein disulfide- isomerase ERp46. Biochem J 453:475-85 (2013). IP, WB ; Human . PubMed: 23713588
- Liu Y et al. Apratoxin a reversibly inhibits the secretory pathway by preventing cotranslational translocation. Mol Pharmacol 76:91-104 (2009). PubMed: 19403701