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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-Taf4 antibody
See all Taf4 products (5) ...
Description
Mouse polyclonal to Taf4
Tested applications
Cross reactivity
Reacts with
Caenorhabditis elegans
Immunogen
Fusion protein: FNNGRTPGPPQPPPPQQQSQQQPPLEMRQIPNPNQIPPQM VQGGPHMVSVGARPMIRPMGPGGPSPMGLQGPVRGPMGHQ MVQMHPPPPPQQIQQQHPAP, corresponding to amino acids 224/323 of Caenorhabditis elegans Taf4
FNNGRTPGPP QPPPPQQQSQ QQPPLEMRQI PNPNQIPPQM VQGGPHMVSV GARPMIRPMG PGGPSPMGLQ GPVRGPMGHQ MVQMHPPPPP QQIQQQHPAP
General notes
Produced from outbred CD1 mice
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Constituents: 50% Glycerol
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
-
Western blot - Taf4 antibody (ab21928)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab21928 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
Application notes
WB: 1/1000. Predicted molecular weight: 60 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Target
Relevance
Taf4 makes part of TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It potentiates transcriptional activation by the AF-2S of retinoic acid, vitamin D3 and thyroid hormone.
Cellular localization
Nuclear
Alternative names
- TAF2C antibody
- TAF2C1 antibody
- TAF4A antibody
see all
Database links
- Entrez Gene: 171630 Caenorhabditis elegans
- SwissProt: O61707 Caenorhabditis elegans
Anti-Taf4 antibody images:
Western blot - Taf4 antibody (ab21928)
All lanes : Anti-Taf4 antibody (ab21928) at 1/1000 dilution
Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a
negative control fusion protein with an irrelevant antigen at 20 ug
Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the
antigen fusion protein at 20 ug
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at
1/5000 dilution
Predicted band size : 60 kDa
The molecular weight of the band on the western blot does not correspond to the predicted band size above (predicted from the molecular weight of the natural protein) because of the additional mass of the fusion and because the fusion protein only contains a partial fragment of the gene.
References for Anti-Taf4 antibody (ab21928)
ab21928 has not yet been referenced specifically in any publications.
Publishing research using ab21928? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"