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ab52846 has not yet been referenced specifically in any publications.
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All lanes : Anti-Taf4 antibody (ab52846) at 1/1000 dilution
Lane 1 : Total protein extract from E coli with ~50ng to 100ng of an irrelevant antigen
Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 110 kDa
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is tagged.
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