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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-Taf4 antibody
See all Taf4 products (5) ...
Description
Mouse polyclonal to Taf4
Tested applications
Cross reactivity
Reacts with
Fruit fly (Drosophila melanogaster)
Immunogen
Fusion protein: MNTSQTAAGN RITFTSQPLP NGTISIAGNP GAVISTAQLP NTTTIKTIQA GIGGQHQGLQ QVHHVQQQQQ SQQQQQQQQQ TQSAGQPLLN SMLPAGVVVG , corresponding to amino acids 695-794 of Fruit fly (Drosophila melanogaster) Taf4
MNTSQTAAGN RITFTSQPLP NGTISIAGNP GAVISTAQLP NTTTIKTIQA GIGGQHQGLQ QVHHVQQQQQ SQQQQQQQQQ TQSAGQPLLN SMLPAGVVVG
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, Whole serum
Concentration
Concentration information loading...
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
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Western blot - Taf4 antibody (ab52846)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52846 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: 1/1000. Predicted molecular weight: 110 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Taf4 makes part of TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It potentiates transcriptional activation by the AF-2S of retinoic acid, vitamin D3 and thyroid hormone.
Cellular localization
Nuclear
Alternative names
- TAF2C antibody
- TAF2C1 antibody
- TAF4A antibody
see all
Database links
- Entrez Gene: 39765 Fruit fly (Drosophila melanogaster)
- SwissProt: P47825 Fruit fly (Drosophila melanogaster)
Anti-Taf4 antibody images:
Western blot - Taf4 antibody (ab52846)
All lanes : Anti-Taf4 antibody (ab52846) at 1/1000 dilution
Lane 1 : Total protein extract from E coli with ~50ng to 100ng of an irrelevant antigen
Lane 2 : Total protein extract from E coli with ~50ng to 500ng of the antigen
Lysates/proteins at 20 µg per lane.
Secondary
Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution
Predicted band size : 110 kDa
The molecular weight of the band on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the gene is used and it is tagged.
References for Anti-Taf4 antibody (ab52846)
ab52846 has not yet been referenced specifically in any publications.
Publishing research using ab52846? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"