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Products:Epigenetics and Nuclear Signaling >> Transcription >> Other factors
Overview
Product name
Anti-Taf4 antibody
See all Taf4 products (5) ...
Description
Rabbit polyclonal to Taf4
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Saccharomyces cerevisiae
Immunogen
Recombinant fragment which was over-expressed in E.coli using a plasmid carrying the N-terminal domain of Taf4 protein (1-200aa) of Saccharomyces cerevisiae and purified by chromatography.
Positive control
Extract of budding yeast cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
-
Western blot - Taf4 antibody (ab63910)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa).
ELISA: Use at an assay dependent dilution.
Target
Relevance
Taf4 makes part of TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It potentiates transcriptional activation by the AF-2S of retinoic acid, vitamin D3 and thyroid hormone.
Cellular localization
Nuclear
Alternative names
- TAF(II)135 antibody
- TAF2C antibody
- TAF2C1 antibody
see all
Database links
- SwissProt: P50105 Saccharomyces cerevisiae
Anti-Taf4 antibody images:
Western blot - Taf4 antibody (ab63910)
All lanes : Anti-Taf4 antibody (ab63910) at 1/5000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Extract of budding yeast
Predicted band size : 42 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Additional bands at : 39 kDa,70 kDa. We are unsure as to the identity of these extra bands.
References for Anti-Taf4 antibody (ab63910)
This product has been referenced in:
- Seizl Met al. Mediator head subcomplex Med11/22 contains a common helix bundle building block with a specific function in transcription initiation complex stabilization. Nucleic Acids Res : (2011). WB; Saccharomyces cerevisiae.Read more (PubMed: 21498544) »
See 1 publication for this product
Publishing research using ab63910? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"