|
|
|
|
Western blot - Taf4 antibody (ab63910)

(enlarge)
|
|
|
|
|
Product Name
|
Taf4 antibody
|
| |
See all Taf4 antibodies (5)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to Taf4
|
|
Immunogen
|
Recombinant fragment which was over-expressed in E.coli using a plasmid carrying the N-terminal domain of Taf4 protein (1-200aa) of Saccharomyces cerevisiae and purified by chromatography.
|
|
Reacts with
(species key)
|
Sc
|
|
Tested applications
(see key)
|
ELISA, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions ELISA: Use at an assay dependent dilution. WB: 1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 42 kDa).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Extract of budding yeast cells.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Transcription >> Transcription Factors Chromatin and Nuclear Signaling >> Transcription >> Other factors
|
|
Relevance
|
Taf4 makes part of TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It potentiates transcriptional activation by the AF-2S of retinoic acid, vitamin D3 and thyroid hormone.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window SwissProt | | P50105 (Saccharomyces cerevisiae)
|
|
|
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Whole antiserum
|
|
Storage buffer
|
Preservative: 0.1% Sodium Azide Constituents: Whole serum Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
Concentration not determined - why is this?
|
|
Storage instructions
|
Store at +4°C.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Taf4 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Taf4 antibody images, references, products related to ab63910 and other tools.
|
| |
Taf4 antibody images:
|
Western blot - Taf4 antibody (ab63910)
All lanes : Taf4 antibody (ab63910) at 1/5000 dilution
Lane 1 : Molecular weight markers Lane 2 : Extract of budding yeast
Predicted band size : 42 kDa Observed band size : 48 kDa (why is the actual band size different from the predicted?) Additional bands at : 39 kDa,70 kDa. We are unsure as to the identity of these extra bands.
|
| |
| |
|
Search PubMed (MEDLINE) for references to Taf4
|
| |
Taf4 antibody - more information
|
Customer reviews (feedback) regarding Taf4 antibody |
Customer FAQs regarding Taf4 antibody |
Protocols for Taf4 antibody |
Price and availability of products related to Taf4 antibody |
| |
Taf4 antibody - related products:
|
|
|
| |
Taf4 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about Taf4 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"