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Anti-Taf4 antibody (ab69949)

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Overview

Product name

Anti-Taf4 antibody
See all Taf4 products (5) ...

Description

Rabbit polyclonal to Taf4

Specificity

ab69949 detects endogenous levels of total Taf4 protein.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide derived from an internal sequence of human Taf4

Positive control

NIH3T3 cell extract.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab69949 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Clonality

Polyclonal

Isotype

IgG

  • Western blot - Taf4 antibody (ab69949)Western blot - Taf4 antibody (ab69949) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab69949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 110 kDa (predicted molecular weight: 110 kDa).

  • ELISA

     ELISA: 1/5000

    ELISA: 1/5000

Target

Relevance

Taf4 makes part of TFIID, a multimeric protein complex that plays a central role in mediating promoter responses to various activators and repressors. It potentiates transcriptional activation by the AF-2S of retinoic acid, vitamin D3 and thyroid hormone.

Cellular localization

Nuclear

Alternative names

  • TAF(II)135 antibody
  • TAF2C antibody
  • TAF2C1 antibody
  • TAF4A antibody
  • TAF4P antibody
  • TAFII 130 antibody
  • TAFII 135 antibody
  • TAFII130 antibody
  • TAFII135 antibody
  • TBP associated factor 4 antibody
  • TBP Associated transcription Factor family member antibody
  • Transcription initiation factor TFIID 135 kDa subunit antibody
  • Transcription initiation factor TFIID subunit 4 antibody
see all

Database links

Anti-Taf4 antibody images:

  Western blot - Taf4 antibody (ab69949)

Western blot - Taf4 antibody (ab69949)

All lanes : Anti-Taf4 antibody (ab69949) at 1/500 dilution

Lane 1 : NIH3T3 cell extract
Lane 2 : NIH3T3 cell extract with immunising peptide at 10 µg

Lysates/proteins at 30 µg per lane.


Predicted band size : 110 kDa
Observed band size : 110 kDa

References for Anti-Taf4 antibody (ab69949)

ab69949 has not yet been referenced specifically in any publications.

Publishing research using ab69949? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"