Anti-Tafazzin / TAZ antibody (ab40004)
Key features and details
- Goat polyclonal to Tafazzin / TAZ
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Tafazzin / TAZ antibody
See all Tafazzin / TAZ primary antibodies -
Description
Goat polyclonal to Tafazzin / TAZ -
Host species
Goat -
Specificity
This antibody is expected to recognise isoforms 1 to 4. (NP_000107.1; NP_851828.1; NP_851829.1; NP_851830.1, respectively). -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog, Chimpanzee -
Immunogen
Synthetic peptide:
C-HLKTQAEQLHNH
(Human) from the C Terminus of the protein sequence (according to NP_000107.1; NP_851828.1; NP_851829.1; NP_851830.1.). -
Positive control
- Recombinant Human Tafazzin / TAZ protein (ab114664) can be used as a positive control in WB. Human heart lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab40004 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Notes |
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WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Some isoforms may be involved in cardiolipin (CL) metabolism. -
Tissue specificity
High levels in cardiac and skeletal muscle. Up to 10 isoforms can be present in different amounts in different tissues. Most isoforms are ubiquitous. Isoforms that lack the N-terminus are found in leukocytes and fibroblasts, but not in heart and skeletal muscle. Some forms appear restricted to cardiac and skeletal muscle or to leukocytes. -
Involvement in disease
Barth syndrome -
Sequence similarities
Belongs to the taffazin family. -
Domain
The hydrophilic domain may serve as an exposed loop interacting with other proteins. -
Cellular localization
Cytoplasm and Membrane. - Information by UniProt
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Database links
- Entrez Gene: 449590 Chimpanzee
- Entrez Gene: 6901 Human
- Entrez Gene: 66826 Mouse
- Entrez Gene: 363521 Rat
- Omim: 300394 Human
- SwissProt: Q6IV84 Chimpanzee
- SwissProt: Q16635 Human
- Unigene: 409911 Human
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Alternative names
- Barth syndrome antibody
- BTHS antibody
- Cardiomyopathy dilated 3A (X linked) antibody
see all
Images
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Anti-Tafazzin / TAZ antibody (ab40004) at 0.3 µg/ml + Human Heart lysate in RIPA buffer at 35 µg
Predicted band size: 33 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Datasheets and documents
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Datasheet download
References (1)
ab40004 has been referenced in 1 publication.
- Gonzalvez F et al. Cardiolipin provides an essential activating platform for caspase-8 on mitochondria. J Cell Biol 183:681-96 (2008). WB ; Human . PubMed: 19001123