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Anti-Tal1 antibody (ab75739)

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Overview

Product name

Anti-Tal1 antibody
See all Tal1 products (3) ...

Description

Rabbit polyclonal to Tal1

Tested applications

IHC-P, WB, ELISAmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Synthetic peptide (Human) corresponding to amino acid residues surrounding S122 conjugated to KLH

Positive control

Mouse heart tissue lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

Purified through a protein A column, followed by peptide affinity purification.

Clonality

Polyclonal

Isotype

IgG

Research areas

Developmental Biology >> Organogenesis >> Hematopoietic system development
Stem Cells >> Hematopoietic Progenitors >> Hemangioblast
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Hematopoietic Progenitors >> Intracellular Molecules
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Differentiation
Cell Biology >> Cell Cycle >> Cell differentiation

  • Western blot - Tal1 antibody (ab75739)Western blot - Tal1 antibody (ab75739) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tal1 antibody (ab75739)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tal1 antibody (ab75739) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab75739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ShowHide1 Image

    WB

     WB: 1/50 - 1/100.Predict...Read more →

    WB: 1/50 - 1/100.Predicted molecular weight: 34 kDa.

  • ELISA

     ELISA: 1/1000

    ELISA: 1/1000

Target

Function

Implicated in the genesis of hemopoietic malignancies. It may play an important role in hemopoietic differentiation. Serves as a positive regulator of erythroid differentiation.

Tissue specificity

Leukemic stem cell.

Involvement in disease

Note=A chromosomal aberration involving TAL1 may be a cause of some T-cell acute lymphoblastic leukemias (T-ALL). Translocation t(1;14)(p32;q11) with T-cell receptor alpha chain (TCRA) genes.

Sequence similarities

Contains 1 basic helix-loop-helix (bHLH) domain.

Domain

The helix-loop-helix domain is necessary and sufficient for the interaction with DRG1.

Post-translational
modifications

Phosphorylated on serine residues. Phosphorylation of Ser-122 is strongly stimulated by hypoxia.
Ubiquitinated; subsequent to hypoxia-dependent phosphorylation of Ser-122, ubiquitination targets the protein for rapid degradation via the ubiquitin system. This process may be characteristic for microvascular endothelial cells, since it could not be observed in large vessel endothelial cells.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP17542 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • bHLHa17 antibody
  • Class A basic helix-loop-helix protein 17 antibody
  • OTTHUMP00000009563 antibody
  • OTTHUMP00000009564 antibody
  • SCL antibody
  • Stem cell protein antibody
  • T cell acute lymphocytic leukemia 1 antibody
  • T cell acute lymphocytic leukemia 1 protein antibody
  • T cell leukemia/lymphoma 5 protein antibody
  • T-cell acute lymphocytic leukemia protein 1 antibody
  • T-cell leukemia/lymphoma protein 5 antibody
  • Tal 1 antibody
  • Tal 1 product antibody
  • TAL 1 protein antibody
  • TAL-1 antibody
  • tal1 antibody
  • TAL1_HUMAN antibody
  • TCL 5 antibody
  • TCL5 antibody
see all

Database links

Anti-Tal1 antibody images:

  Western blot - Tal1 antibody (ab75739)

Western blot - Tal1 antibody (ab75739)

Anti-Tal1 antibody (ab75739) at 1/50 dilution + Mouse heart tissue lysate at 35 µg

Predicted band size : 34 kDa
Observed band size : 40 kDa (why is the actual band size different from the predicted?)
Additional bands at : 28 kDa. We are unsure as to the identity of these extra bands.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tal1 antibody (ab75739)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tal1 antibody (ab75739)

ab75739 at 1/100 dilution staining Tal1 in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.

References for Anti-Tal1 antibody (ab75739)

This product has been referenced in:

  • Tsunoda Tet al. Immune-related zinc finger gene ZFAT is an essential transcriptional regulator for hematopoietic differentiation in blood islands. Proc Natl Acad Sci U S A 107:14199-204 (2010). IHC-P; Mouse.Read more (PubMed: 20660741) »

See 1 publication for this product

Publishing research using ab75739? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"