Anti-Talin 1 and 2 antibody [8D4] (ab11188)
Key features and details
- Mouse monoclonal [8D4] to Talin 1 and 2
- Suitable for: IP, Flow Cyt, ICC/IF, WB
- Reacts with: Rat, Chicken, Human, Xenopus laevis, Chinese hamster
- Isotype: IgG1
Overview
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Product name
Anti-Talin 1 and 2 antibody [8D4] -
Description
Mouse monoclonal [8D4] to Talin 1 and 2 -
Host species
Mouse -
Tested applications
Suitable for: IP, Flow Cyt, ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Chicken, Human, Xenopus laevis, Chinese hamster
Does not react with: Fish -
Immunogen
Full length native protein (purified) corresponding to Chicken Talin 1 and 2.
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Positive control
- WB: CHO cells ICC/IF: Chicken UMNSAH/DF1 cells
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General notes
This product was changed from ascites to tissue culture supernatant on 25th October 2016. The following lot(s) is/are from ascites and is still in stock as of 25th October 2016- GR228677. Lot numbers higher than GR228677 will be from tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly.
For continuous use, store at 2-8 °C for up to one month. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Proprietary Purification -
Purification notes
Purified from Tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
8D4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11188 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use at an assay dependent concentration.
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Flow Cyt | (1) |
Use at an assay dependent concentration.
See Abreview. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF | (1) |
Use at an assay dependent concentration.
See Abreview. |
WB | (2) |
Use at an assay dependent concentration. Predicted molecular weight: 270 kDa.
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Notes |
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IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. See Abreview. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. See Abreview. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 270 kDa. |
Target
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Relevance
Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin. Talin and vinculin have been shown to interact with each other and both have been proposed to be involved in generating the transmembrane connection, between the extracellular matrix and the cytoskeleton, that occurs at adhesion plaques. At physiological ionic strength, talin is an elongate, flexible, monomeric protein with the ability to self-associate into dimers at higher protein concentrations. Talin 2 is related to talin 1. This protein has a different pattern of expression compared to talin 1 but, like talin 1, is thought to associate with unique transmembrane receptors to form novel linkages between extracellular matrices and the actin cytoskeleton. -
Cellular localization
Cell projection, ruffle membrane (talin 1), Focal adhesion plaques and synapses (talin 2) -
Database links
- Entrez Gene: 395194 Chicken
- Entrez Gene: 7094 Human
- Entrez Gene: 83660 Human
- Entrez Gene: 313494 Rat
- Omim: 186745 Human
- Omim: 607349 Human
- SwissProt: P54939 Chicken
- SwissProt: Q8AWI0 Chicken
see all -
Alternative names
- talin 1 antibody
- talin 2 antibody
- talin antibody
see all
Images
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Immunocytochemistry analysis of Chicken UMNSAH/DF1 cells labeling Talin 1 and 2 with ab11188 at 5 μg/mL. Cells were fixed and permeabilized with methanol followed by aceton. The antibody was developed using Goat Anti-Mouse IgG Atto-488 conjugate.
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Lane 1 : Anti-Talin 1 and 2 antibody [8D4] (ab11188) at 2 µg/ml
Lane 2 : Anti-Talin 1 and 2 antibody [8D4] (ab11188) at 1 mg/ml
All lanes : Whole extract of CHO cells
Secondary
All lanes : Goat Anti-Mouse IgG-Peroxidase
Predicted band size: 270 kDachemiluminescent substrate.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (16)
ab11188 has been referenced in 16 publications.
- Kao SH et al. Surface Viscosity-Dependent Neurite Initiation in Cortical Neurons. Adv Biol (Weinh) 6:e2101325 (2022). PubMed: 35362269
- Marhuenda E et al. Glioma stem cells invasive phenotype at optimal stiffness is driven by MGAT5 dependent mechanosensing. J Exp Clin Cancer Res 40:139 (2021). PubMed: 33894774
- Liu Q et al. Anesthetic Propofol Promotes Tumor Metastasis in Lungs via GABAA R-Dependent TRIM21 Modulation of Src Expression. Adv Sci (Weinh) 8:e2102079 (2021). PubMed: 34263559
- Son K et al. The eosinophil actin cytoskeleton undergoes rapid rearrangement in response to fluid shear stress. J Leukoc Biol N/A:N/A (2020). PubMed: 32349177
- Saleh A et al. A novel 3D nanofibre scaffold conserves the plasticity of glioblastoma stem cell invasion by regulating galectin-3 and integrin-ß1 expression. Sci Rep 9:14612 (2019). PubMed: 31601895