Products:Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Talin / Moesin
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ab11188 has been referenced in 3 publications.
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Central Panel: ab11188 staining Talin 1 and 2 in human PMN cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.1% TritonX-100 + 2% BSA in PBS and blocked with 2% BSA for 1 hour at 22ºC. Samples were incubated with primary antibody 1/100 in blocking buffer for 4 hours at 37ºC. An Alexa Fluor® 488-conjugated Goat polyclonal to mouse IgG, dilution 1/100, was used as secondary antibody.
Left-hand panel: Nuclei counterstained with DAPI (blue).
Right-hand panel: Overlay
This image is courtesy of an Abreview submitted by Dr. Mahesh Shivananjappa
ab11188 staining Talin 1 and 2 in Human platelet cells by Flow cytometry.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton-X-100 in 2% BSA for 15 minutes. Primary antibody used at a 1/250 dilution and incubated for 18 hours at 4°C. The secondary antibody used was an Alexa Fluor®488 conjugated goat anti-mouse IgG (H+L) at a 1/500 dilution.
Image courtesy of Dr Mahesh Shivananjappa by Abreview.
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