Western blot - Tankyrase antibody [19A449] (ab13587)

Western blot analysis of Tankyrase in HeLa cells with 1ug/ml of ab13587.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tankyrase antibody [19A449] (ab13587)

ab13587 at 1/150 staining mouse small intestine tissue sections by IHC-P. The tissue was paraformaldeyde fixed and a heat mediated antigen retrieval step was performed in citrate buffer before incubation with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence-Tankyrase antibody [19A449](ab13587)

ICC/IF image of ab13587 stained Mcf7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab13587, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Flow Cytometry - Tankyrase antibody [19A449] (ab13587)

Overlay histogram showing HEK293 cells stained with ab13587 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13587, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.