Anti-Tankyrase antibody [19A449] (ab13587)

in the data sheet of ab13587 you show a western blot analysis of Tankyrase in HeLa cells. Which protocol did you use and are these HeLa cells overepressing the Tankyrase? Thanks a lot for your support in advance,

ANSWER:

Thank you for your enquiry. A standard protocol was used to run the Western blot. The cells used were HeLa growing in culture (native proliferating HeLa, e.g., not over expressing recombinant Tankyrase). If you have any further questions then please get back in contact with me.

Ab13587 - states that the antibody detects a band of approximately 120 kDa. However, when you look at SwissProt, it states that the MW is approx. 142 kDa.

Ab2184 - states that this antibody recognizes an endogenous band at ~150 kDa in whole cell extract.

Customer says that Tankyrase 2 is approx 126 kDa, and Tankyrase 1 is approx 142 kDa.

So, is ab13587 specific for Tankyrase 1 or 2?

ANSWER:

Thank you again for your phone call. The originator of ab2184 has told me that the antibody is specific for Tankyrase 1.

As I mentioned, I'm sending you ab2184 free of charge and it is on order# 88192 which you should receive later this week. Please let me know how it works out for you and if you have any additional questions or concerns.

Ab13587 - states that the antibody detects a band of approximately 120 kDa. However, when you look at SwissProt, it states that the MW is approx. 142 kDa.

Ab2184 - states that this antibody recognizes an endogenous band at ~150 kDa in whole cell extract.

Customer says that Tankyrase 2 is approx 126 kDa, and Tankyrase 1 is approx 142 kDa.

So, is ab13587 specific for Tankyrase 1 or 2?

ANSWER:

Thank you for your enquiry and patience. Regarding ab13587, I have obtained some clarification from the antibody's originator.

Ab13587 was developed in 1998, before the 2 forms were discovered. The accession number of the sequence used as the immunogen is Genbank accession number AAC79841 and this accession number is now known to correspond to Tankyrase 1. Tankyrase 1 is a 1327 amino acid protein. However, Tankyrase I and Tankyrase 2 share high sequence homology. The epitope of the antibody has not been mapped. We know for sure that it recognizes Tankyrase 1 (as the antibody was made against full length Tankyrase 1). However, there is a good chance that it also recognizes Tankyrase 2 due to the high sequence homology.

It was stated that this antibody detects a band at approximately 120 kDa. However, the 120 kDa designation was based on the mini blot observation- which is above the 116 kDa; it was just an approximation. Thus, it is hard to say if it 126 Kda or 142 kDa.

Regarding ab2184, I believe that the antibody recognizes Tankyrase 1 as well, but I'm still waiting for clarification from the antibody's originator. Once I hear, I will let you know.

If you have any additional questions or concerns, please let me know.