Anti-Tbx3 antibody (ab113275)

Overview

• Product nameAnti-Tbx3 antibodySee all Tbx3 primary antibodies ...
• Description
  Rabbit polyclonal to Tbx3
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide, corresponding to a region within amino acids 400-450 of Human Tbx3 (NP_057653.3).

• Positive controlHeLa and 293T whole cell lysates.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium azide
  Constituent: 99% Tris citrate/phosphate
  Note: pH 7 to 8
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab113275 was affinity purified using an epitope specific to Tbx3 immobilized on solid support.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Organogenesis
  • Angiogenesis and vasculogenesis

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Developmental Families
  • Other

Applications

Target

• FunctionTranscriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation.
• Tissue specificityWidely expressed.
• Involvement in diseaseDefects in TBX3 are the cause of ulnar-mammary syndrome (UMS) [MIM:181450]. UMS is characterized by ulnar ray defects, obesity, hypogenitalism, delayed puberty, hypoplasia of nipples and apocrine glands.
• Sequence similaritiesContains 1 T-box DNA-binding domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession O15119 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6926 Human
  • Omim: 601621 Human
  • SwissProt: O15119 Human
  • Unigene: 129895 Human

• Alternative names
  Bladder cancer related protein XHL antibodyT box 3 antibodyT-box protein 3 antibody

  T-box transcription factor TBX3 antibodyTbx3 antibodyTBX3 ISO antibodyTBX3_HUMAN antibodyUMS antibodyXHL antibody

  see all

Anti-Tbx3 antibody images

• 

  Western blot - Tbx3 antibody (ab113275)
  All lanes : Anti-Tbx3 antibody (ab113275) at 0.4 µg/ml
  
  Lane 1 : HeLa whole cell lysate at 50 µg
  Lane 2 : HeLa whole cell lysate at 15 µg
  Lane 3 : HeLa whole cell lysate at 5 µg
  Lane 4 : 293T whole cell lysate at 50 µg
  
  
  Predicted band size : 79 kDa
  
  
  Exposure time : 3 minutes
• 

  Immunoprecipitation - Tbx3 antibody (ab113275)
  Detection of Tbx3 by Western Blot of Immunprecipitate.
  ab113275 at 1µg/ml staining Tbx3 in HeLa whole cell lysate immunoprecipitated using ab113275 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
  Detection: Chemiluminescence with exposure time of 3 minutes.