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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other
Overview
Product name
Anti-Tbx3 antibody
See all Tbx3 products (6) ...
Description
Rabbit polyclonal to Tbx3
Specificity
Ab66306 is specific for isoform 3 of Tbx3.
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
A region within synthetic peptide: CRDKGSPAVK AHLFAAERPR DSGRLDKASP DSRHSPATIS SSTRGLGAEE, corresponding to internal sequence amino acids 404-453 of Human Tbx3
CRDKGSPAVK AHLFAAERPR DSGRLDKASP DSRHSPATIS SSTRGLGAEE
Positive control
HepG2 cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
-
Western blot - Tbx3 antibody (ab66306)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent concentration. (PubMed: 21341990)
WB: Use a concentration of 1.25 µg/mlPredicted molecular weight: 66 kDa.(Specific for isoform 3 of Tbx3.)
Target
Function
Transcriptional repressor involved in developmental processes. Probably plays a role in limb pattern formation.
Tissue specificity
Widely expressed.
Involvement in disease
Defects in TBX3 are the cause of ulnar-mammary syndrome (UMS) [MIM:181450]. UMS is characterized by ulnar ray defects, obesity, hypogenitalism, delayed puberty, hypoplasia of nipples and apocrine glands.
Sequence similarities
Contains 1 T-box DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO15119
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Bladder cancer related protein XHL antibody
- T box 3 antibody
- T-box protein 3 antibody
see all
Database links
- Entrez Gene: 781063 Cow
- Entrez Gene: 477494 Dog
- Entrez Gene: 6926 Human
- Entrez Gene: 21386 Mouse
- Entrez Gene: 353305 Rat
- NCBI: NP_932169.1 Mouse
- Omim: 601621 Human
- SwissProt: O15119 Human
see all
Anti-Tbx3 antibody images:
Western blot - Tbx3 antibody (ab66306)
Anti-Tbx3 antibody (ab66306) at 1.25 µg/ml (in 5% skim milk / PBS buffer) + HepG2 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 66 kDa
Gel concentration: 12%. Ab66306 is specific for isoform 3 of Tbx3.
References for Anti-Tbx3 antibody (ab66306)
This product has been referenced in:
- Yano Tet al. Tara up-regulates E-cadherin transcription by binding to the Trio RhoGEF and inhibiting Rac signaling. J Cell Biol 193:319-32 (2011).Read more (PubMed: 21482718) »
- Bozyk PDet al. Mesenchymal stromal cells from neonatal tracheal aspirates demonstrate a pattern of lung-specific gene expression. Stem Cells Dev 20:1995-2007 (2011). ICC/IF; Human.Read more (PubMed: 21341990) »
See all 2 publications for this product
Publishing research using ab66306? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"