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Anti-Tbx6 antibody (ab30946)

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Overview

Product name

Anti-Tbx6 antibody
See all Tbx6 products (3) ...

Description

Rabbit polyclonal to Tbx6

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide derived from a region between amino acids 268-317 of human Tbx6. (Note: exact amino acid sequence is proprietary).

Positive control

Jurkat cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS.

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

This antibody has been purified by peptide affinity chromatography method.

Clonality

Polyclonal

Isotype

IgG

Research areas

Developmental Biology >> Reproduction >> Germ cell markers

Stem Cells >> Neural Stem Cells >> Intracellular
Stem Cells >> Germline Stem Cells >> Embryonic Germ Cells
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> Developmental Families >> Other

  • Western blot - Tbx6 antibody (ab30946)Western blot - Tbx6 antibody (ab30946) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab30946 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/312500

    ELISA: 1/312500

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1.25 µg/mlDetects a band of approximately 42 kDa (predicted molecular weight: 47 kDa).

Target

Function

Probable transcriptional regulator involved in developmental processes. Could be required for specification of paraxial mesoderm structures during gastrulation.

Tissue specificity

Expressed in fetal tail bud, posterior spinal tissue, intervertebral disk and testis. Also expressed in adult testis, kidney, lung, muscle and thymus.

Sequence similarities

Contains 1 T-box DNA-binding domain.

Developmental stage

Expressed during gastrulation and during a second phase in some adult tissues.

Cellular localization

Nucleus.

Target information above from: UniProt accessionO95947 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DFNB 67 antibody
  • DFNB67 antibody
  • T box 6 antibody
  • T box protein 6 antibody
  • T box transcription factor TBX 6 antibody
  • T box transcription factor TBX6 antibody
  • T-box protein 6 antibody
  • T-box transcription factor TBX6 antibody
  • Tbox 6 antibody
  • TBX 6 antibody
  • tbx6 antibody
  • TBX6_HUMAN antibody
see all

Database links

Anti-Tbx6 antibody images:

  Western blot - Tbx6 antibody (ab30946)

Western blot - Tbx6 antibody (ab30946)

Anti-Tbx6 antibody (ab30946) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

Secondary
HRP conjugated anti rabbit IgG at 1/50000 dilution

Predicted band size : 47 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)

References for Anti-Tbx6 antibody (ab30946)

ab30946 has not yet been referenced specifically in any publications.

Publishing research using ab30946? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"