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Does not react withMouse, Rat
Synthetic peptide corresponding to residues in the C terminus of human Telomerase reverse transcriptase.
Our Abpromise guarantee covers the use of ab32020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 127 kDa).|
|ICC/IF||1/50 - 1/100.|
|IP||Use at an assay dependent dilution.|
|IHC-P||Use at an assay dependent dilution. PubMed: 20367640|
|IHC-Fr||Use a concentration of 0.75 µg/ml. PubMed: 17982423|
ab32020 staining Telomerase reverse transcriptase in human thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% hydrogen peroxide for 10 minutes at 25°C; antigen retrieval was by heat mediation in citrate buffer pH6.0 at 100°C for 20 minutes. Samples were incubated with primary antibody (1/100) for 20 minutes at 25°C. An undiluted HRP polymer-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.
ab32020 staining Telomerase in human breast carcinoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton x100 and blocking with 2% serum was performed for 30 minutes in 370C. Samples were incubated with primary antibody (1/100) in PBS for 1 hour 30 minutes at 37°C. An Alexa Fluor® 594-conjugated goat monoclonal to rabbit IgG was used at dilution at 1/75 as secondary antibody.
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