RabMAb

Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)

Overview

  • Product nameAnti-Telomerase reverse transcriptase antibody [Y182]See all Telomerase reverse transcriptase primary antibodies ...
  • Description
    Rabbit monoclonal [Y182] to Telomerase reverse transcriptase
  • Tested applicationsWB, ICC/IF, Flow Cyt, IP, IHC-P, IHC-Fr more details
  • Species reactivity
    Reacts with: Cow, Human

    Does not react with

    Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to residues in the C terminus of human Telomerase reverse transcriptase.

  • Positive control
    • WB: Hela cell lysate. IHC-P: Human lung adenocarcinoma.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab32020 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000. Detects a band of approximately 122 kDa (predicted molecular weight: 127 kDa).
ICC/IF 1/50 - 1/100.
Flow Cyt 1/100.
IP Use at an assay dependent dilution.
IHC-P Use at an assay dependent dilution. PubMed: 20367640
IHC-Fr Use a concentration of 0.75 µg/ml. PubMed: 17982423
  • Application notesIs unsuitable for ICC.
  • Target

    • FunctionTelomerase is a ribonucleoprotein enzyme essential for the replication of chromosome termini in most eukaryotes. Active in progenitor and cancer cells. Inactive, or very low activity, in normal somatic cells. Catalytic component of the teleromerase holoenzyme complex whose main activity is the elongation of telomeres by acting as a reverse transcriptase that adds simple sequence repeats to chromosome ends by copying a template sequence within the RNA component of the enzyme. Catalyzes the RNA-dependent extension of 3'-chromosomal termini with the 6-nucleotide telomeric repeat unit, 5'-TTAGGG-3'. The catalytic cycle involves primer binding, primer extension and release of product once the template boundary has been reached or nascent product translocation followed by further extension. More active on substrates containing 2 or 3 telomeric repeats. Telomerase activity is regulated by a number of factors including telomerase complex-associated proteins, chaperones and polypeptide modifiers. Modulates Wnt signaling. Plays important roles in aging and antiapoptosis.
    • Tissue specificityExpressed at a high level in thymocyte subpopulations, at an intermediate level in tonsil T lymphocytes, and at a low to undetectable level in peripheral blood T lymphocytes.
    • Involvement in diseaseNote=Activation of telomerase has been implicated in cell immortalization and cancer cell pathogenesis.
      Defects in TERT are associated with susceptibilty to aplastic anemia (AA) [MIM:609135]. AA is a rare disease in which the reduction of the circulating blood cells results from damage to the stem cell pool in bone marrow. In most patients, the stem cell lesion is caused by an autoimmune attack. T-lymphocytes, activated by an endogenous or exogenous, and most often unknown antigenic stimulus, secrete cytokines, including IFN-gamma, which would in turn be able to suppress hematopoiesis.
      Note=Genetic variations in TERT are associated with coronary artery disease (CAD).
      Defects in TERT are a cause of dyskeratosis congenita autosomal dominant (ADDKC) [MIM:127550]; also known as dyskeratosis congenita Scoggins type. ADDKC is a rare, progressive bone marrow failure syndrome characterized by the triad of reticulated skin hyperpigmentation, nail dystrophy, and mucosal leukoplakia. Early mortality is often associated with bone marrow failure, infections, fatal pulmonary complications, or malignancy.
      Defects in TERT are a cause of susceptibility to pulmonary fibrosis idiopathic (IPF) [MIM:178500]. Pulmonary fibrosis is a lung disease characterized by shortness of breath, radiographically evident diffuse pulmonary infiltrates, and varying degrees of inflammation and fibrosis on biopsy. It results in acute lung injury with subsequent scarring and endstage lung disease.
    • Sequence similaritiesBelongs to the reverse transcriptase family. Telomerase subfamily.
      Contains 1 reverse transcriptase domain.
    • DomainThe primer grip sequence in the RT domain is required for telomerase activity and for stable association with short telomeric primers.
      The RNA-interacting domain 1 (RD1)/N-terminal extension (NTE) is required for interaction with the pseudoknot-template domain of each of TERC dimers. It contains anchor sites that bind primer nucleotides upstream of the RNA-DNA hybrid and is thus an essential determinant of repeat addition processivity.
      The RNA-interacting domain 2 (RD2) is essential for both interaction with the CR4-CR5 domain of TERC and for DNA sythesis.
    • Post-translational
      modifications
      Ubiquitinated, leading to proteasomal degradation.
      Phosphorylation at Tyr-707 under oxidative stress leads to translocation of TERT to the cytoplasm and reduces its antiapoptotic activity. Dephosphorylated by SHP2/PTPN11 leading to nuclear retention. Phosphorylation by the AKT pathway promotes nuclear location.
    • Cellular localizationNucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Chromosome > telomere. Cytoplasm. Nucleus > PML body. Shuttling between nuclear and cytoplasm depends on cell cycle, phosphorylation states, transformation and DNA damage. Diffuse localization in the nucleoplasm. Enriched in nucleoli of certain cell types. Translocated to the cytoplasm via nuclear pores in a CRM1/RAN-dependent manner involving oxidative stress-mediated phosphorylation at Tyr-707. Dephosphorylation at this site by SHP2 retains TERT in the nucleus. Translocated to the nucleus by phosphorylation by AKT.
    • Target information above from: UniProt accession O14746 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • EST2 antibody
      • hEST2 antibody
      • htert antibody
      • TCS1 antibody
      • Telomerase associated protein 2 antibody
      • Telomerase Catalytic Subunit antibody
      • Telomerase Catalytic Subunit antibody
      • Telomerase reverse transcriptase antibody
      • Telomerase-associated protein 2 antibody
      • Telomere Reverse Transcriptase antibody
      • Telomere Reverse Transcriptase antibody
      • TERT antibody
      • TERT antibody
      • TERT_HUMAN antibody
      • TP2 antibody
      • TRT antibody
      • TRT antibody
      see all

    Anti-Telomerase reverse transcriptase antibody [Y182] images

    • Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020) at 1/1000 dilution + Hela cell lysate

      Predicted band size : 127 kDa
      Observed band size : 122 kDa (why is the actual band size different from the predicted?)
    • Ab32020, at a 1/50 dilution, staining Telomerase Reverse Transcriptase in HeLa cells by Immunofluorescence.
    • ab32020 staining Telomerase in human breast carcinoma cells by Immunocytochemistry/ Immunofluorescence. Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton x100 and blocking with 2% serum was performed for 30 minutes in 370C. Samples were incubated with primary antibody (1/100) in PBS for 1 hour 30 minutes at 37°C. An Alexa Fluor® 594-conjugated goat monoclonal to rabbit IgG was used at dilution at 1/75 as secondary antibody.

      See Abreview

    References for Anti-Telomerase reverse transcriptase antibody [Y182] (ab32020)

    This product has been referenced in:
    • Diaz A  et al. TERT and AURKA gene copy number gains enhance the detection of acral lentiginous melanomas by fluorescence in situ hybridization. J Mol Diagn 16:198-206 (2014). IHC . Read more (PubMed: 24374110) »
    • Jung AR  et al. Increased alternative lengthening of telomere phenotypes of telomerase-negative immortal cells upon trichostatin--a treatment. Anticancer Res 33:821-9 (2013). WB ; Human . Read more (PubMed: 23482750) »

    See all 17 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
    Sample Marmoset (common) Tissue sections (Adult testis)
    Specification Adult testis
    Permeabilization No
    Fixative Formaldehyde
    Username

    Zachary Yu-Ching Lin

    Verified customer

    Submitted Jun 21 2013

    Thank you for contacting us.

    Unfortunately we do not have any telomerase antibodies tested and known to cross-react with dog. However, we have some telomerase and telomerase reverse transcriptase antibodies that showed some sequence homology...

    Read More

    Thank you for contacting us. Yes, this is the same product. Epitomics is now part of Abcam so you will see the same products in our catalog from theirs. Please let me know if you have any further questions.

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (corneal endothelium)
    Specification corneal endothelium
    Fixative Formaldehyde
    Permeabilization No
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 37°C
    Username

    Dr. Zhiguo He

    Verified customer

    Submitted Nov 01 2012

    Gracias por tu respuesta.

    Siento mucho que no consiguierais solucionar los problemas de tinción del anticuerpo. No hay ningún problema en mandaros de forma gratuita el anticuerpo ab5181. Este anticuerpo se ha validado en IHC-P (...

    Read More

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.
    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has c...

    Read More

    Thank you very much for your interest in our antibodies.

    As an Oct4 antibody, I can recommend the ab19857 for use in ChIP. It has been tested to workgoodin ChIP andto recognize thehuman, mouse and rhesus monkey protein. http://www.abcam.com/i...

    Read More

    Vielen Dank für Ihre Anfrage.

    LotGR3691-2 von ab32020 hat eine Konzentration von 0.33mg/ml.

    Ich möchte Sie darauf hinweisen, dass dieser Antikörper ist ein monoklonaler Antikörper aus dem Kaninchen. Kaninchen pro...

    Read More

    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

    Read More

    Gracias por tu respuesta.

    Me temo que nosotros no ofrecemos ese servicio de testeo de anticuerpos con muestras de otros laboratorios.

    Si te parece, podemos enviaros de forma totalmente gratuita, un nuevo vial de anticuerpo, bien del...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"