Tenascin C antibody [BC-24] (ab6393)

Product Name 

Tenascin C antibody [BC-24]

Product type 

Primary antibodies

Description 

Mouse monoclonal [BC-24] to Tenascin C

Immunogen 

Full length native protein (purified) (Human).

Reacts with 
(species key)

Hu

Specificity 

BC-24 reacts specifically with tenascin using ELISA, RIA and immunoblotting procedures.
The product reacts with tenascin from frozen sections as well as protease-digested formalin-fixed, paraffin-embedded, Bouin's-, Brunnel's-, B5-, alcohol-acetic acid- and Susa solution-fixed tissues.
The antibody recognizes all isoforms of human tenascin.

Epitope 

BC-24 recognizes an epitope located within the N-terminal EGF-like sequence of the human tenascin molecule.

Tested applications 
(see key)

Dot, ELISA, ICC, IHC-FoFr, IHC-Fr, IHC-P, RIA, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependent dilution.
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
IHC-P: 1/4000. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution.
RIA: Use at an assay dependent dilution.
WB: 1/200.


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Tenascin is synthesized by fibroblasts, chondroblasts, osteocytes, smooth muscle cells and glial cells.

Cellular localization 

Secreted; extracellular matrix.

Research areas 

Stem Cells >> Mesenchymal Stem Cells >> Secreted Molecules
Stem Cells >> Neural Stem Cells >> Extracellular
Neuroscience >> Neurology process >> Neural Signal Transduction
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Tenascin
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins

Relevance 

Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle.

Tenascin (TN)(Erickson and Bourdon 1989, Erickson 1993) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1-200/220, myotendinous antigen (MI), neuronectin (NEC1) and glioma mesenchymal extracellular matrix (GMEM). The TN molecule is a disulfide-linked hexamer. Human TN has 3 subunits of 190, 200 and 220 kDa. It is primarily made up of 14.5 epidermal growth-factor-like repeats, 15 units similar to the fibronectin type-III-homology repeat and, at the C-terminus, has a sequence with homology to the globular domain of the beta and gamma chain of fibrinogen.
Monoclonal antibody reacting specifically with tenascin is an essential tool for the localization, identification and studies on the role of the molecule in epithelial-mesenchymal and neuronal-glial interactions (Castellucci et al. 1991, Balza et al. 1993).

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

BC-24

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Ascitic fluid with 0.1% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 7.40 - 7.90 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

Find concentration of your lot :

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

References for Tenascin C antibody [BC-24] (ab6393)

  This product has been used in: (two most recent references)

Esposito I et al. Autoimmune pancreatocholangitis, non-autoimmune pancreatitis and primary sclerosing cholangitis: a comparative morphological and immunological analysis. PLoS ONE 3:e2539 (2008). IHC-P; Human. PubMed: 18596913

Fiorilli P et al. Integrins mediate adhesion of medulloblastoma cells to tenascin and activate pathways associated with survival and proliferation. Lab Invest 88:1143-56 (2008). PubMed: 18794852

See all 6 publication references for this product.


If you publish research using ab6393 please let us know so that we can cite the reference on this datasheet.

Customer reviews (feedback) regarding Tenascin C antibody [BC-24]

Customer FAQs regarding Tenascin C antibody [BC-24]

Protocols for Tenascin C antibody [BC-24]

Price and availability of products related to Tenascin C antibody [BC-24]

Tenascin C antibody [BC-24] - related products: