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Products:Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Overview
Product name
Anti-Tenascin C antibody [BC-24]
See all Tenascin C products (11) ...
Description
Mouse monoclonal [BC-24] to Tenascin C
Specificity
BC-24 reacts specifically with tenascin using ELISA, RIA and immunoblotting procedures. The product reacts with tenascin from frozen sections as well as protease-digested formalin-fixed, paraffin-embedded, Bouin's-, Brunnel's-, B5-, alcohol-acetic acid- and Susa solution-fixed tissues. The antibody recognizes all isoforms of human tenascin.
Tested applications
WB, ELISA, IHC-FoFr, IHC-P, RIA, IHC-Fr, ICC, Dot Blotmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length native protein (purified) (Human).
Epitope
BC-24 recognizes an epitope located within the N-terminal EGF-like sequence of the human tenascin molecule.
Positive control
Tenascin is synthesized by fibroblasts, chondroblasts, osteocytes, smooth muscle cells and glial cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Primary antibody notes
Tenascin (TN)(Erickson and Bourdon 1989, Erickson 1993) is a high molecular weight, multifunctional, extracellular matrix glycoprotein, expressed in association with mesenchymal-epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1-200/220, myotendinous antigen (MI), neuronectin (NEC1) and glioma mesenchymal extracellular matrix (GMEM). The TN molecule is a disulfide-linked hexamer. Human TN has 3 subunits of 190, 200 and 220 kDa. It is primarily made up of 14.5 epidermal growth-factor-like repeats, 15 units similar to the fibronectin type-III-homology repeat and, at the C-terminus, has a sequence with homology to the globular domain of the beta and gamma chain of fibrinogen. Monoclonal antibody reacting specifically with tenascin is an essential tool for the localization, identification and studies on the role of the molecule in epithelial-mesenchymal and neuronal-glial interactions (Castellucci et al. 1991, Balza et al. 1993).
Clonality
Monoclonal
Clone number
BC-24
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Secreted Molecules
Stem Cells >> Neural Stem Cells >> Extracellular
Neuroscience >> Neurology process >> Neural Signal Transduction
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Tenascin
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab6393 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
WB: 1/200WB: 1/200
ELISA: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution.
IHC-P: 1/4000Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
RIA: Use at an assay dependent dilution.
IHC-Fr: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
Dot: Use at an assay dependent dilution.
Target
Function
Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
Sequence similarities
Belongs to the tenascin family.
Contains 15 EGF-like domains.
Contains 1 fibrinogen C-terminal domain.
Contains 15 fibronectin type-III domains.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP24821
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 150 225 antibody
- Cytotactin antibody
- Glioma associated extracellular matrix antigen antibody
see all
Database links
- Entrez Gene: 3371 Human
- Omim: 187380 Human
- SwissProt: P24821 Human
- Unigene: 143250 Human
References for Anti-Tenascin C antibody [BC-24] (ab6393)
This product has been referenced in:
- Mahesparan Ret al. Expression of extracellular matrix components in a highly infiltrative in vivo glioma model. Acta Neuropathol 105:49-57 (2003). ICC; Human, Rat.Read more (PubMed: 12471461) »
- Palumbo Cet al. Peripheral nerve extracellular matrix remodeling in Charcot-Marie-Tooth type I disease. Acta Neuropathol 104:287-96 (2002). IHC; Human.Read more (PubMed: 12172915) »
See all 9 publications for this product
Publishing research using ab6393? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"