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ab6346 has been referenced in 10 publications.
Publishing research using ab6346? Please let us know so that we can cite the reference in this datasheet
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ab6346 staining Tenascin C in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed Frozen sections). Tissue samples were fixed with paraformaldehyde and permeabilized with 1% Triton X100 before blocking with 10% serum for 30 minutes at 250C. The sample was incubated with primary antibody (1/100) for 16 hours at 250C in 10% NGS in PBS + 0.1% TX100. An Alexa Fluor®488-conjugated Goat polyclonal to rat IgG was used as secondary antibody at dilution at 1/400.
This image is a courtesy of Ben Deverman
IHC image of Tenascin C staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6346, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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