You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-Tenascin C antibody [MTn-12] (ab6346)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-Tenascin C antibody [MTn-12]
See all Tenascin C products (11) ...

Description

Rat monoclonal [MTn-12] to Tenascin C

Specificity

MTn-12 (Aufderheide et al. 1988) reacts specifically with mouse tenascin. In supernatants of cultured mouse fibroblasts, the antibody precipitates two polypeptides of ~210 and ~260 kDa. It also recognizes these tenascin chains and the 230 kDa endoglycosidase F-digested fragment in immunoblotting of tissue extracts and fibroblast lysates under reducing conditions (Aufderheide et al. 1988, Weller et al. 1991). In ELISA, it was shown that the antibody reacts specifically against mouse tenascin, but not against fibronectin and collagen type I. Immunoprecipitation data suggests that molecules of the expected size for tenascin-C are detected by MTn-12, and not other tenascins. One can expect several bands in blots or immunoprecipitations but this is because the antibody can detect several splice variants of tenascin C. Nevertheless, the mapping of the epitope and the other data cannot entirely exclude the possibility that it reacts with other tenascins. This would have to be tested directly with purified tenascins.

Tested applications

WB, IP, ELISA, IHC-Fr, IF, Dot Blot, IHC-P, IHC-FoFrmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Full length native protein (partially purified) (Mouse).

Epitope

Binding site has been mapped to the 7-8 fibronectin type III-like repeats of recombinant fragments of mouse tenascin-C

Positive control

for WB: tenascin immunoprecipitated from supernatants of metabolically labeled fibroblasts (Aufderheide and Ekblom), confluent mouse mammary epithelial cells cell lines NOG-8, EpH4, Fos-ER and 410.4, HC-11) (Wirl et al), Purified tenascin (Weller et al).

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 15mM Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Monoclonal

Clone number

MTn-12

Myeloma

unknown

Isotype

IgG1

Light chain type

unknown

Research areas

Stem Cells >> Mesenchymal Stem Cells >> Secreted Molecules
Stem Cells >> Neural Stem Cells >> Extracellular
Neuroscience >> Neurology process >> Neural Signal Transduction
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tenascin C antibody [MTn-12] (ab6346)Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tenascin C antibody [MTn-12] (ab6346) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tenascin C antibody [MTn-12] (ab6346)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tenascin C antibody [MTn-12] (ab6346) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab6346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.

Sequence similarities

Belongs to the tenascin family.
Contains 15 EGF-like domains.
Contains 1 fibrinogen C-terminal domain.
Contains 15 fibronectin type-III domains.

Cellular localization

Secreted > extracellular space > extracellular matrix.

Target information above from: UniProt accessionP24821 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 150 225 antibody
  • Cytotactin antibody
  • Glioma associated extracellular matrix antigen antibody
  • Glioma-associated-extracellular matrix antigen antibody
  • GMEM antibody
  • GP 150 225 antibody
  • GP 150-225 antibody
  • GP antibody
  • Hexabrachion antibody
  • HXB antibody
  • JI antibody
  • MGC167029 antibody
  • Miotendinous antigen antibody
  • Myotendinous antigen antibody
  • Neuronectin antibody
  • TENA_HUMAN antibody
  • Tenascin antibody
  • Tenascin-C antibody
  • Tenascin-C isoform 14/AD1/16 antibody
  • TenascinC antibody
  • TN antibody
  • TN C antibody
  • TN-C antibody
  • TNC antibody
see all

Database links

Anti-Tenascin C antibody [MTn-12] images:

  Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tenascin C antibody [MTn-12] (ab6346)

Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Tenascin C antibody [MTn-12] (ab6346)

ab6346 staining Tenascin C in mouse brain tissue section by Immunohistochemistry (PFA perfusion fixed Frozen sections). Tissue samples were fixed with paraformaldehyde and permeabilized with 1% Triton X100 before blocking with 10% serum for 30 minutes at 250C. The sample was incubated with primary antibody (1/100) for 16 hours at 250C in 10% NGS in PBS + 0.1% TX100. An Alexa Fluor®488-conjugated Goat polyclonal to rat IgG was used as secondary antibody at dilution at 1/400.

This image is a courtesy of Ben Deverman

See Abreview

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tenascin C antibody [MTn-12] (ab6346)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tenascin C antibody [MTn-12] (ab6346)

IHC image of Tenascin C staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6346, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

References for Anti-Tenascin C antibody [MTn-12] (ab6346)

This product has been referenced in:

  • Chiao YAet al. In vivo matrix metalloproteinase-7 substrates identified in the left ventricle post-myocardial infarction using proteomics. J Proteome Res 9:2649-57 (2010). WB; Mouse.Read more (PubMed: 20232908) »
  • Sotiropoulou PAet al. Bcl-2 and accelerated DNA repair mediates resistance of hair follicle bulge stem cells to DNA-damage-induced cell death. Nat Cell Biol 12:572-82 (2010).Read more (PubMed: 20473297) »

See all 9 publications for this product

Publishing research using ab6346? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"