Tenascin C antibody [MTn-12] (ab6346)

Product Name 

Tenascin C antibody [MTn-12]

Product type 

Primary antibodies

Description 

Rat monoclonal [MTn-12] to Tenascin C

Immunogen 

Full length native protein (partially purified) (Mouse).

Reacts with 
(species key)

Ms

Specificity 

MTn-12 (Aufderheide et al. 1988) reacts specifically with mouse tenascin. In supernatants of cultured mouse fibroblasts, the antibody precipitates two polypeptides of ~210 and ~260 kDa. It also recognizes these tenascin chains and the 230 kDa endoglycosidase F-digested fragment in immunoblotting of tissue extracts and fibroblast lysates under reducing conditions (Aufderheide et al. 1988, Weller et al. 1991).

In ELISA, it was shown that the antibody reacts specifically against mouse tenascin, but not against fibronectin and collagen type I.

Immunoprecipitation data suggests that molecules of the expected size for tenascin-C are detected by MTn-12, and not other tenascins. One can expect several bands in blots or immunoprecipitations but this is because the antibody can detect several splice variants of tenascin C. Nevertheless, the mapping of the epitope and the other data cannot entirely exclude the possibility that it reacts with other tenascins. This would have to be tested directly with purified tenascins.

Epitope 

Binding site has been mapped to the 7-8 fibronectin type III-like repeats of recombinant fragments of mouse tenascin-C

Tested applications 
(see key)

Dot, ELISA, ICC/IF, IHC-FoFr, IHC-Fr, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Dot: Use at an assay dependant dilution.
ELISA: Use at an assay dependant dilution.
IF: 1/200 for staining of unfixed, frozen tissue sections of mouse intestine (also see reference Wirl et al).
IHC-Fr: Use at an assay dependant dilution.
IHC-FoFr: 1/100.
IP: Use at a concentration of 50 µg/ml in 200 µl Tris-buffered saline (TBS)(see reference Wirl et al).
WB: 1/20 (see references Wirl et al and Weller et al). Block with 0.1% gelatine in PBS (see Wirl et al).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

for WB: tenascin immunoprecipitated from supernatants of metabolically labeled fibroblasts (Aufderheide and Ekblom), confluent mouse mammary epithelial cells cell lines NOG-8, EpH4, Fos-ER and 410.4, HC-11) (Wirl et al), Purified tenascin (Weller et al).

Cellular localization 

Secreted; extracellular matrix.

Research areas 

Stem Cells >> Mesenchymal Stem Cells >> Secreted Molecules
Stem Cells >> Neural Stem Cells >> Extracellular
Neuroscience >> Neurology process >> Neural Signal Transduction
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins

Relevance 

Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle.

Database links 

Raised in 

Rat

Clonality 

Monoclonal

Clone number 

MTn-12

Isotype 

IgG1

Purity 

Ascites

Storage buffer 

Ascitic fluid with 0.1% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Batch dependent within range: 5.00 - 40.40 mg/ml

Concentration of lot no.     is  

Concentration not available for this lot.

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Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Tenascin C antibody [MTn-12] images, references, products related to ab6346 and other tools.

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References for Tenascin C antibody [MTn-12] (ab6346)

  This product has been used in: (two most recent references)

Yasui Y et al. A primary hepatic malignant mesenchymal tumor with myofibrogenic differentiation in a B6C3F1 mouse. Toxicol Pathol 37:244-8 (2009). PubMed: 19332665

Bazigou E et al. Integrin-alpha9 is required for fibronectin matrix assembly during lymphatic valve morphogenesis. Dev Cell 17:175-86 (2009). IHC-FoFr, htPCR; Mouse. PubMed: 19686679

See all 9 publication references for this product.


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