Tenascin C antibody [T2H5] (ab3970)

Product Name 

Tenascin C antibody [T2H5]

Product type 

Primary antibodies

Description 

Mouse monoclonal [T2H5] to Tenascin C

Immunogen 

Full length native protein (purified from a homogenate of a mammary tumour specimen) (Human).

Reacts with 
(species key)

Hu

Tested applications 
(see key)

IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-P: Use at a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use at an assay dependant dilution.
WB: Use at an assay dependant dilution. Detects a band of approximately 240 kDa.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human tonsil tissue

Cellular localization 

Secreted; extracellular matrix.

Research areas 

Stem Cells >> Mesenchymal Stem Cells >> Secreted Molecules
Stem Cells >> Neural Stem Cells >> Extracellular
Neuroscience >> Neurology process >> Neural Signal Transduction
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Tenascin
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins

Relevance 

Tenascin, also known as hexabrachion and cytotactin, is an extracellular matrix protein with a spatially and temporally restricted tissue distribution. It is a hexameric, multidomain protein with disulfide linked subunits of 190 to 240 kD, originally characterized as 'myotendinous antigen.' In the embryo it is present in dense mesenchyme surrounding developing epithelia and in developing cartilage and bone. In the adult, tenascin remains present in tendons and myotendinous junctions in the perichondrium and periosteum, as well as in smooth muscle.

Tenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

T2H5

Isotype 

IgG1

Purity 

>95% by SDS-PAGE

Storage buffer 

Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

See below for Tenascin C antibody [T2H5] images, references, products related to ab3970 and other tools.

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References for Tenascin C antibody [T2H5] (ab3970)

  This product has been used in: (two most recent references)

Gulubova M Immunohistochemical localization of collagen type III and type IV, laminin, tenascin and alpha-smooth muscle actin (alphaSMA) in the human liver in peliosis. Pathol Res Pract 198:803-12 (2002). PubMed: 12608657

Ioachim E et al. Immunohistochemical expression of extracellular matrix components tenascin, fibronectin, collagen type IV and laminin in breast cancer: their prognostic value and role in tumour invasion and progression. Eur J Cancer 38:2362-70 (2002). PubMed: 12460779

See all 4 publication references for this product.


If you publish research using ab3970 please let us know so that we can cite the reference on this datasheet.

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