![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tenascin C antibody [T2H5] (ab3970) image](#ab3970_1.jpg)
![Western blot - Tenascin C antibody [T2H5] (ab3970) image](#Tenascin-C-Primary-antibodies-ab3970-2.jpg)
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Products:Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Overview
Product name
Anti-Tenascin C antibody [T2H5]
See all Tenascin C products (11) ...
Description
Mouse monoclonal [T2H5] to Tenascin C
Tested applications
IHC-P, IP, WB, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Full length native protein (purified from a homogenate of a mammary tumour specimen) (Human).
Positive control
Human tonsil tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 15mM Sodium Azide
Constituents: PBS, pH 7.4
Concentration
Concentration information loading...
Purity
>95% by SDS-PAGE
Purification notes
Purified from hybridoma culture supernatant by protein A-affinity chromatography.
Primary antibody notes
Tenascin is a high molecular weight, multifunctional, hexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development and in the neovasculature and stroma of undifferentiated tumors. It has been described under a variety of names: cytotactin, hexabrachion protein, J1, myotendinous antigen (MI) and glioma mesenchymal extracellular matrix (GMEM). The tenascin molecule is a disulfide-linked hexamer. The expression of tenascin is associated with development and growth, both normal and pathological, whereas the distribution in normal adult tissue is restricted.
Clonality
Monoclonal
Clone number
T2H5
Myeloma
unknown
Isotype
IgG1
Light chain type
unknown
Research areas
Stem Cells >> Mesenchymal Stem Cells >> Secreted Molecules
Stem Cells >> Neural Stem Cells >> Extracellular
Neuroscience >> Neurology process >> Neural Signal Transduction
Signal Transduction >> Cytoskeleton / ECM >> Extracellular Matrix >> ECM Proteins >> Tenascin
Neuroscience >> Cell Adhesion Proteins >> ECM Proteins
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3970 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 10 µg/mlPerform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Detects a band of approximately 240 kDa.
ICC/IF: Use at an assay dependent dilution. (PubMed: 20435685)
Target
Function
Extracellular matrix protein implicated in guidance of migrating neurons as well as axons during development, synaptic plasticity as well as neuronal regeneration. Promotes neurite outgrowth from cortical neurons grown on a monolayer of astrocytes. Ligand for integrins alpha-8/beta-1, alpha-9/beta-1, alpha-V/beta-3 and alpha-V/beta-6.
Sequence similarities
Belongs to the tenascin family.
Contains 15 EGF-like domains.
Contains 1 fibrinogen C-terminal domain.
Contains 15 fibronectin type-III domains.
Cellular localization
Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accessionP24821
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- 150 225 antibody
- Cytotactin antibody
- Glioma associated extracellular matrix antigen antibody
see all
Database links
- Entrez Gene: 3371 Human
- Omim: 187380 Human
- SwissProt: P24821 Human
- Unigene: 143250 Human
Anti-Tenascin C antibody [T2H5] images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tenascin C antibody [T2H5] (ab3970)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Tenascin C antibody [T2H5] (ab3970)](/ps/datasheet/Images/3/ab3970/ab3970_1.jpg)
ab3970 staining paraffin embedded tissue sections.
Western blot - Tenascin C antibody [T2H5] (ab3970)
![Western blot - Tenascin C antibody [T2H5] (ab3970)](/ps/datasheet/images/3/ab3970/Tenascin-C-Primary-antibodies-ab3970-2.jpg)
Anti-Tenascin C antibody [T2H5] (ab3970) at 1 µg/ml + U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 ��g
Secondary
Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed (ab97040) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 265 kDa (why is the actual band size different from the predicted?)
Additional bands at : 34 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes
Tenascin C contains an exstensive number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
References for Anti-Tenascin C antibody [T2H5] (ab3970)
This product has been referenced in:
- Singh SRet al. Can lineage-specific markers be identified to characterize mesenchyme-derived cell populations in the human airways? Am J Physiol Lung Cell Mol Physiol 299:L169-83 (2010). ICC/IF; Human.Read more (PubMed: 20435685) »
- Xiong Wet al. Tenascin-C as a prognostic biomarker in osteosarcoma? Chin Med J (Engl) 122:2737-43 (2009). IHC-P; Human.Read more (PubMed: 19951606) »
See all 6 publications for this product
Publishing research using ab3970? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"