Overview

  • Product nameThioredoxin (TrxR) Reductase Assay KitSee all Thioredoxin reductase kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Assay time
    0h 40m
  • Product overview

    Abcam's Thioredoxin Reductase Assay Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the assay TrxR catalyzes the reduction of 5, 5'-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generate a strong yellow color (λmax = 412 nm). Since in crude biological samples other enzymes, such as glutathione reductase and glutathione peroxidase, can also reduce DTNB, therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed: the first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Thioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide.

  • Tested applicationsFunctional Studiesmore details

Properties

  • RelevanceThioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide.
  • Cellular localizationTXNRD1: Cytoplasmic. TXNRD2: Cytoplasmic. Nuclear. Microsome. Endoplasmic reticulum. TXNRD3: Mitochondrial.
  • Alternative names
    • EC 1.8.1.9
    • gene associated with retinoic and interferon-induced mortality 12 protein
    • Gene associated with retinoid IFN induced mortality 12 protein
    • GRIM 12
    • KM 102 derived reductase like factor
    • KM-102-derived reductase-like factor
    • MGC9145
    • oxidoreductase
    • Selenoprotein Z
    • SELZ
    • TGR
    • Thioredoxin and glutathione reductase
    • Thioredoxin glutathione reductase
    • Thioredoxin reductase 1
    • thioredoxin reductase 1, cytoplasmic
    • Thioredoxin reductase 2
    • thioredoxin reductase 2, mitochondrial
    • Thioredoxin reductase 3
    • Thioredoxin reductase beta
    • Thioredoxin reductase GRIM 12
    • thioredoxin reductase TR1
    • thioredoxin reductase TR2
    • thioredoxin reductase TR3
    • TR
    • TR beta
    • TR1
    • TR2
    • TR3
    • TrxR
    • TRXR1
    • TRXR2
    • TRXR3
    • TXNR
    • TXNRD1
    • TXNRD2
    • TXNRD3
    see all

Applications

Our Abpromise guarantee covers the use of ab83463 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution.

Thioredoxin (TrxR) Reductase Assay Kit images

  • TNB (Thionitrobenzoic acid) measured in mouse tissue lysates after 25 min. incubation showing quantity (mU) per mg protein of tested sample.

  • TNB (Thionitrobenzoic acid) measured in mouse tissue lysates showing quantity (nmol) per mg protein of tested sample

  • TNB (Thionitrobenzoic acid) measured in cell lysates after 25 min. showing quantity (mU) per 1 mln of tested cells

  • Standard curve (colourimetric) : mean of duplicates (+/-SD) with background readings substracted

  • TNB (Thionitrobenzoic acid) measured in cell lysates showing quantity (nmol) per 1 mln of tested cells

  • Thioredoxin reductase Kinetic Data using ab83463.
  • TNB Standard Curve using ab83463.

Protocols

References for Thioredoxin (TrxR) Reductase Assay Kit (ab83463)

This product has been referenced in:
  • Sampson N  et al. ROS Signaling by NOX4 Drives Fibroblast-to-Myofibroblast Differentiation in the Diseased Prostatic Stroma. Mol Endocrinol 25:503-15 (2011). Read more (PubMed: 21273445) »

See 1 Publication for this product

Product Wall

Ab83463 can be used to measure TrxR in plasma. It does not need any special preparation. It can be used directly or diluted depending on the TrxR content.

Several volumes must be tested in a pilot assay to ensure readings are within the linear...

Read More

I wanted to let you know that I have been informed by the lab that there is EDTA in this kit.

Thank you for contacting us.

The standard in this kit is from rat.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for contacting us.

I can confirm, that regrettably we can not provide any data using serum samples with this kit. However, every sample type and application stated for a product on our datasheets is covered by our six month guarantee...

Read More

Thank you for contacting us and your interest in our products.

I can confirm this product is sourced externally and the molar concentration of the TrxR inhibitor is proprietary information. Regrettably we are not allowed to chare this informat...

Read More

I have had some feedback from the lab to share with you which may help to improve the results seen so far.


The assay buffer needs to be allowed to come to room temperature before use. This may have significantly reduced the reactivity o...

Read More

Thank you for providing that information.

Could I ask you confirm a few additional details?

1. How was the kit stored for the month in which it was not used?

2. In particular, how was the NADPH kept, both before reconstitu...

Read More

Thank you for contacting us and reporting the problems experienced with the Thioredoxin (TrxR) Reductase Assay Kit (ab83463). From having spoken to you over the phone, there does not immediately appear to be an obvious solution to what you have been ob...

Read More

Thank you for contacting us. The Thioredoxin (TrxR) Reductase Assay Kit ab83463 contains a TrxR specific inhibitor that is utilized to determine TrxR specific activity. Unfortunately, we have not tested the kit or the inhibitor with insect cells. ...

Read More

Thank you for contacting us.  The Thioredoxin (TrxR) Reductase Assay Kit ab83463 contains a TrxR specific inhibitor that is utilized to determine TrxR specific activity. Unfortunately, we have not tested the kit or the inhibitor with insect cells. ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"