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Thioredoxin (TrxR) Reductase Assay Kit (ab83463)

Q&A (7)Specific References (1)

Overview

  • Product nameThioredoxin (TrxR) Reductase Assay Kit
  • Tests
    100 x 1 assay
  • Sample type
    Cell culture supernatant, Urine, Other biological fluids, Tissue Extracts
  • Assay typeEnzyme activity
  • Assay time
    0h 40m
  • Product overview

    Abcam's Thioredoxin Reductase Assay Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the assay TrxR catalyzes the reduction of 5, 5'-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2-), which generate a strong yellow color (λmax = 412 nm). Since in crude biological samples other enzymes, such as glutathione reductase and glutathione peroxidase, can also reduce DTNB, therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed: the first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.
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  • Notes

    Thioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide.

  • Tested applicationsFunctional Studies more details

Properties

  • RelevanceThioredoxin reductase (TrxR) (EC 1.8.1.9) is a ubiquitous enzyme which is involved in many cellular processes such as cell growth, p53 activity, and protection against oxidation stress, etc. The mammalian TrxR reduces thioredoxins as well as non-disulfide substrates such as selenite, lipoic acids, lipid hydroperoxides, and hydrogen peroxide.
  • Cellular localizationTXNRD1: Cytoplasmic. TXNRD2: Cytoplasmic. Nuclear. Microsome. Endoplasmic reticulum. TXNRD3: Mitochondrial.
    • Alternative names
        EC 1.8.1.9Gene associated with retinoid IFN induced mortality 12 proteinGRIM 12
        KM 102 derived reductase like factorMGC9145Selenoprotein ZSELZTGRThioredoxin and glutathione reductaseThioredoxin glutathione reductaseThioredoxin reductase 1Thioredoxin reductase 2Thioredoxin reductase 3Thioredoxin reductase betaThioredoxin reductase GRIM 12TRTR betaTR1TR2TR3TrxRTRXR1TRXR2TRXR3TXNRTXNRD1TXNRD2TXNRD3
      see all

    Applications

    Our Abpromise guarantee covers the use of ab83463 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies FuncS

    Thioredoxin (TrxR) Reductase Assay Kit images

    • Functional Studies - Thioredoxin Reductase Assay Kit (ab83463)
      Functional Studies - Thioredoxin Reductase Assay Kit (ab83463)
      TNB Standard Curve using ab83463.
    • Functional Studies - Thioredoxin Reductase Assay Kit (ab83463)
      Functional Studies - Thioredoxin Reductase Assay Kit (ab83463)
      Thioredoxin reductase Kinetic Data using ab83463.

    Protocols

    References for Thioredoxin (TrxR) Reductase Assay Kit (ab83463)

    This product has been referenced in:
    • Sampson N  et al. ROS Signaling by NOX4 Drives Fibroblast-to-Myofibroblast Differentiation in the Diseased Prostatic Stroma. Mol Endocrinol 25:503-15 (2011). Read more (PubMed: 21273445) »

    See 1 Publication for this product

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    Displaying 1 - 7 of 7 results for Abreviews and Q&A

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    Inquiry: Hi, I would like to use the thioredoxin reductase assay kit with serum. The kit protocol suggests that serum is a suitable sample. I was wondering if you have any data to show that thioredoxin activity can be measured from serum using this kit. M...

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    Thank you for contacting us.

    I can confirm, that regrettably we can not provide any data using serum samples with this kit. However, every sample type and application stated for a product on our datasheets is covered by our six month guarantee. I...

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    Hello!



    Would it be possible to find out the molar concentration of the TrxR inhibitor that comes with the Thioredoxin Reductase Assay Kit ab83463?



    Thanks for your help!



    Best regards,

    Thank you for contacting us and your interest in our products.

    I can confirm this product is sourced externally and the molar concentration of the TrxR inhibitor is proprietary information. Regrettably we are not allowed to chare this information...

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    Hello,
    Thank you for your response. I recieved the kit in october and then did not use it for around a month and then used it throughout November (sorry I do not have the exact date of delivery) The order number of the kit is GR56486-1.
    Sample -...

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    I have had some feedback from the lab to share with you which may help to improve the results seen so far.


    The assay buffer needs to be allowed to come to room temperature before use. This may have significantly reduced the reactivity obse...

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    Hello,
    Thank you for your response. I recieved the kit in october and then did not use it for around a month and then used it throughout November (sorry I do not have the exact date of delivery) The order number of the kit is GR56486-1.
    Sample -...

    Read More

    Thank you for providing that information.

    Could I ask you confirm a few additional details?

    1. How was the kit stored for the month in which it was not used?

    2. In particular, how was the NADPH kept, both before reconstitutio...

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    Phone call reporting problems in detection of reduction using the Thioredoxin (TrxR) Reductase Assay Kit in frozen samples.

    Thank you for contacting us and reporting the problems experienced with the Thioredoxin (TrxR) Reductase Assay Kit (ab83463). From having spoken to you over the phone, there does not immediately appear to be an obvious solution to what you have been obser...

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    Our customer wants to use this ab83463 but he will use insect cell, so could you confirm he can use insect cell sample?

    Thank you for contacting us. The Thioredoxin (TrxR) Reductase Assay Kit ab83463 contains a TrxR specific inhibitor that is utilized to determine TrxR specific activity. Unfortunately, we have not tested the kit or the inhibitor with insect cells. The...

    Read More

    Our customer wants to use this kit using insect cell, so please let me know he can use insect cell or not.

    Thank you for contacting us.  The Thioredoxin (TrxR) Reductase Assay Kit ab83463 contains a TrxR specific inhibitor that is utilized to determine TrxR specific activity. Unfortunately, we have not tested the kit or the inhibitor with insect cells. The...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"