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Thrombin-Antithrombin Complex (TAT) Human ELISA Kit (ab108907)

Submit an Abreview Q&A (14)

Overview

  • Product nameThrombin-Antithrombin Complex (TAT) Human ELISA KitSee all Thrombin-Antithrombin Complex kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Milk, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    = 1.5 ng/ml
  • Range
    1.48 ng/ml - 120 ng/ml
  • Recovery

    99 %

  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Human
  • Product overview

    Thrombin-antithrombin (TAT) complexes formed following the neutralization of thrombin by antithrombin III (AT) have been used as a surrogate marker for thrombin generation. High plasma level of TAT complexes has been suggested to alter hemostatic activation in argentine hemorrhagic fever, chronic dialysis patients, and toxemia of pregnancy. Whereas low plasma level of TAT complexes is found in type 1 (insulin-dependent) diabetes, neonatal respiratory distress syndrome, and primary untreated cancer. TAT complexes are a useful marker to predict morphological changes in chronic aortic dissection.

    ab108907 Thrombin-antithrombin Complex Human ELISA kit is designed for detection of human TAT complexes in plasma, milk, and cell culture supernatants. This assay employs a quantitative sandwich enzyme immunoassay technique that measures TAT complexes in less than 4 hours. A monoclonal antibody specific for Antithrombin has been pre-coated onto a microplate. TAT complexes in standards and samples are sandwiched by the immobilized antibody and biotinylated polyclonal antibody specific for Thrombin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.

  • Tested applicationsELISA more details

Properties

  • RelevanceThrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Antithrombin: Most important serine protease inhibitor in plasma that regulates the blood coagulation cascade. Antithrombin inhibits thrombin, matriptase-3/TMPRSS7, as well as factors IXa, Xa and XIa. Its inhibitory activity is greatly enhanced in the presence of heparin.
  • Cellular localizationSecreted
    • Alternative names
        antithrombin IIIAT3AT3D
        ATIIIcoagulation factor II (thrombin)F2PRO0309prothrombinprothrombin B chainPTRPRGL2serine (or cysteine) proteinase inhibitor, clade C (antithrombin), member 1serine proteaseserine-cysteine proteinase inhibitor clade C member 1serpin C1serpin peptidase inhibitor, clade C (antithrombin), member 1SERPINC1TATTHPH1THPH7
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab108907 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      Thrombin-Antithrombin Complex (TAT) Human ELISA Kit images

      • ELISA - Thrombin-antithrombin Complex Human ELISA Kit (ab108907)
        ELISA - Thrombin-antithrombin Complex Human ELISA Kit (ab108907)
        Human Thrombin-antithrombin (TAT) Complexes Standard Curve

      References for Thrombin-Antithrombin Complex (TAT) Human ELISA Kit (ab108907)

      ab108907 has not yet been referenced specifically in any publications.

      Publishing research using ab108907 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab108907 Submit a Question for ab108907

      Displaying 1 - 10 of 14 results for Abreviews and Q&A

      10
      Votes: Highest First

      I was wondering if you could give me an expiration date for the TAT kits (ab108907)



      All our kits are guaranteed for the first six months. But having said that, stored at the right conditions, the reagents could last up to a year.

      How should the standard be diluted to produce the standard curve?





      TAT Complexes Standard: Reconstitute the 240 ng of human TAT Complexes Standard with 2 ml of MIX Diluent to generate a standard solution of 120 ng/ml. Allow the standard to sit for 10 minutes with gentle agitation prior to making dilu...

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      What is the catalogue number of Thrombin-Antithrombin Complex (TAT) Human ELISA Kit?

      Thank you for contacting us.

      The catalogue number is ab108907

      http://www.abcam.com/Thrombin-Antithrombin-Complex-TAT-Human-ELISA-Kit-ab108907.html

      Please find attached the datasheet.

      I hope this information is helpful t...

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      Hi tech support!

      Would it be possible for you to help one of our customers out with reference to the following question please:

      We purchased the Thrombin-Antithrombin Complex (TAT) Human ELISA Kit (ab108907) and was unable to find th...

      Read More

      Thank you for contacting us.

      I am sorry you could not find this information in the protocol book. The range for this kit is stated on our datasheet and corresponds to 0.48 ng/ml - 120 ng/ml.

      I hope this helps and if we can assist furth...

      Read More

      We purchased the Thrombin-Antithrombin Complex (TAT) Human ELISA Kit (ab108907) and was unable to find the normal reference range in the booklet provided nor the website. Could you please provide us with the normal reference range for this ELISA Kit. We r...

      Read More


      The range for the strandard is from 1.48 to 120 ng/ml, as shown on pages 8 and 9 of the booklet: http://www.abcam.com/ps/products/108/ab108907/documents/ab108907%20Thrombin%20Antithrombin%20Complex%20Human%20ELISA%20Kit%20(Web)%20v2.pdf.

      I h...

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      Hello, I would like to know if your thrombin-antithrombin complex ELISA kit, catalogue # ab108907, can be used for mouse plasma. Thank you in advance.

      Thank you for contacting Abcam.
      Unfortunately ab108907 will not work well with mouse samples. We do have a few other kits that work on mouse that may be suitable though:
      http://www.abcam.com/Antithrombin-III-Mouse-ELISA-Kit-ab108800.html
      http:...

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      We tried the TAT kit today doing a half-plate (6 strips), and this looks fine for a first attempt – Standard curve attached. All seems very straightforward to use.
      We will run the other half tomorrow.
      I have frozen the remaining stock stan...

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      Thank you for your reply which has been forwarded to me as my colleague Karin is currently away from the office.

      I am pleased to hear the kit has worked well for you. In answer to your questions:

      1. Yes, as stated in the protocol the...

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      can whole blood (including blood cells) be used?
      have bovine samples been tested?

      Thank you for your patience.

      I have heard back from the lab with the following information:

      We have not yet tested the kit in whole blood. However, we tested bovine samples. The cross-reactivity is very low, around 2%.

      Thus...

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      Phone call querying the quantity of standard provided with the kit (label states 240 mg).

      Thank you for contacting us today.

      I tried calling back this afternoon to let you know what I have found out about the standard provided with the Thrombin-Antithrombin Complex (TAT) Human ELISA Kit (ab108907). I am afraid we have made an error i...

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      What is the normal range for TAT?

      Thank you for contacting us.
      The range the kit can detect is 1.48 ng/ml - 120 ng/ml.
      The amount in a specific sample depends on the sample, and whether a dilution is necessary or not (so that the readings will be within the standard curve) would...

      Read More
      

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"