Overview

  • Product nameAnti-Thrombin antibody
    See all Thrombin primary antibodies
  • Description
    Rabbit polyclonal to Thrombin
  • SpecificityThe antibody will recognize prothrombin and thrombin
  • Tested applicationsIHC-P, Enzyme Immunoassay, RIA, IP, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length native protein: Human thrombin purified from human plasma

  • Positive control
    • IHC-P: Human liver FFPE tissue sections.

Properties

Applications

Our Abpromise guarantee covers the use of ab48626 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Enzyme Immunoassay Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.

Target

  • RelevanceThrombin is an active enzyme in the earliest steps of the blood clot formation, generated from its circulating inactive precursor prothrombin. Thrombin is a glycoprotein formed by two peptides chains of 36 and 259 amino acids linked by disulfure bonds. Three important sites have been identified on the surface of the enzyme: The catalytic site that confers to the molecule its serine protease activity, the exosite one responsible for the binding of the substrate (fibrinogen or thrombin receptor) and the exosite two responsible for the binding of antithrombin III and inactivation of thrombin. The molecular mass of thrombin is 37 kDa.
  • Cellular localizationSecreted
  • Database links
  • Alternative names
    • Coagulation factor II antibody
    • Coagulation factor II thrombin antibody
    • EC 3.4.21.5 antibody
    • F2 antibody
    • Factor II antibody
    • Prothombin antibody
    • Prothrombin B chain antibody
    • PT antibody
    • Serine protease antibody
    see all

Anti-Thrombin antibody images

  • IHC image of Thrombin staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica Bond  system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab48626, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab48626 stained HepG2 cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48626, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Thrombin antibody (ab48626)

ab48626 has not yet been referenced specifically in any publications.

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

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Thank you for contacting me again. Detecting thrombin specifically is a very hard issue. As thrombin is part of pro thrombin and the proform undergoes activation during blood coagulation therefore it is difficult to differentiate both proteins....

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