Thrombopoietin Human ELISA Kit (ab100656)
- Product nameThrombopoietin Human ELISA KitSee all Thrombopoietin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 25 pg/ml
- Range24.69 pg/ml - 6000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.82 84% - 105% Serum 92.21 78% - 101% Plasma 93.44 80% - 103%
- Species reactivityReacts with: Human
- Product overview
Human Thrombopoietin (TPO), also known as megakaryocyte growth and development factor (MGDF) is 60-70 kDa. It is believed to play a key role in the development of megakaryocytes. Thrombopoietin seems to stimulate an increase in megakaryocyte size and number.
ab100656 Human Thrombopoietin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human Thrombopoietin in serum, plasma, cell culture supernatants. This assay employs an antibody specific for human Thrombopoietin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thrombopoietin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human Thrombopoietin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted into the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thrombopoietin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 700X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent A . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody Thrombopoietin: biotinylated anti-human Thrombopoietin 2 vials Stop Solution Item I 1 x 8ml Thrombopoietin Microplate (12 strips x 8 wells) coated with anti-Human Thrombopoietin 1 unit Thrombopoietin Standard Human (recombinant) 2 vials TMB One-Step Substrate Reagent Item H 1 x 12ml
- C mpl ligandMegakaryocyte colony stimulating factorMegakaryocyte growth and development factor
- Megakaryocyte stimulating factorMGC163194MGDFMKCSFMLMPL ligandMPLLGMyeloproliferative leukemia virus oncogene ligandOTTHUMP00000210504THPOTPO
Our Abpromise guarantee covers the use of ab100656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Thrombopoietin Human ELISA Kit (ab100656)
ab100656 has not yet been referenced specifically in any publications.