Thrombopoietin Mouse ELISA Kit (ab100748)
- Product nameThrombopoietin Mouse ELISA KitSee all Thrombopoietin kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 30 pg/ml
- Range27.4 pg/ml - 20000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 96.23 83% - 103% Serum 93.45 82% - 103% Plasma 94.28 84% - 104%
- Species reactivityReacts with: Mouse
- Product overview
The ab100748 Thrombopoietin Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse Thrombopoietin in serum, plasma and cell culture supernates. This assay employs an antibody specific for mouse Thrombopoietin coated on a 96-well plate. Standards and samples are pipetted into the wells and Thrombopoietin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse Thrombopoietin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Thrombopoietin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 300X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent A . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium) diluent. 1 x 15ml Detection Antibody TPO: biotinylated anti-mouse TPO 2 vials Stop Solution Item I 1 x 8ml Thrombopoietin Microplate (12 strips x 8 wells) coated with anti-mouse Thrombopoietin 1 unit Thrombopoietin Standard Mouse (recombinant) 2 vials TMB One-Step Substrate Reagent Item H 1 x 12ml
- Research Areas
- C mpl ligandMegakaryocyte colony stimulating factorMegakaryocyte growth and development factor
- Megakaryocyte stimulating factorMGC163194MGDFMKCSFMLMPL ligandMPLLGMyeloproliferative leukemia virus oncogene ligandOTTHUMP00000210504THPOTPO
- SwissProt: P40226 Mouse
Our Abpromise guarantee covers the use of ab100748 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Thrombopoietin Mouse ELISA Kit (ab100748)
ab100748 has not yet been referenced specifically in any publications.