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Products:Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Overview
Product name
Anti-Thrombopoietin antibody
See all Thrombopoietin products (9) ...
Description
Sheep polyclonal to Thrombopoietin
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse
Immunogen
Recombinant full length protein (Mouse)
Properties
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 0.1M Sodium chloride, 0.05M Sodium phosphate, 1mM EDTA, pH 6.6
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Cell Biology >> Cell Cycle >> Cell differentiation
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
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Western blot - Thrombopoietin antibody (ab62774)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab62774 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/50000ELISA: 1/50000
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 89 kDa (predicted molecular weight: 38 kDa).
Target
Function
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Involvement in disease
Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
Sequence similarities
Belongs to the EPO/TPO family.
Domain
Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.
Cellular localization
Secreted.
Target information above from: UniProt accessionP40225
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- c mpl ligand antibody
- C-mpl ligand antibody
- Megakaryocyte colony stimulating factor antibody
see all
Database links
- Entrez Gene: 21832 Mouse
- SwissProt: P40226 Mouse
- Unigene: 3943 Mouse
Anti-Thrombopoietin antibody images:
Western blot - Thrombopoietin antibody (ab62774)
All lanes : Anti-Thrombopoietin antibody (ab62774) at 1 µg/ml
Lane 1 : 100ng mouse
Thrombopoietin (non-reduced)
Lane 2 : 100ng mouse
Thrombopoietin (reduced)
Secondary
ABC Alkaline Phosphatase Kit
Predicted band size : 38 kDa
Observed band size : 89 kDa, this is larger than the predicted MW and is due to glycosylation.
References for Anti-Thrombopoietin antibody (ab62774)
ab62774 has not yet been referenced specifically in any publications.
Publishing research using ab62774? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"