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Products:Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Overview
Product name
Anti-Thrombopoietin antibody
See all Thrombopoietin products (9) ...
Description
Goat polyclonal to Thrombopoietin
Tested applications
Indirect ELISA, Sandwich ELISA, WB, Neutralisingmore details
Cross reactivity
Reacts with
Human
Immunogen
Highly pure (>98%) recombinant Human full length protein
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cell cycle >> Cell differentiation
Stem Cells >> Mesenchymal Stem Cells >> Osteogenesis
Cell Biology >> Cell Cycle >> Cell differentiation
Cardiovascular >> Blood >> Fibrinolysis / Thrombolysis
Applications
Show applications key
Our Abpromise guarantee covers the use of ab84446 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
I-ELISA: Use a concentration of 0.5 - 2 µg/ml.(detection limit for recombinant Human Thrombopoietin: 0.2 - 0.4 ng/well.)
sELISA: Use a concentration of 0.5 - 2 µg/ml. Can be paired for Sandwich ELISA with Goat polyclonal to Thrombopoietin (Biotin) (ab84445). (Use as capture antibody with recommended pair. Detection limit for recombinant Human Thrombopoietin: 0.2 - 0.4 ng/well. )
WB: Use a concentration of 0.1 - 0.2 µg/ml.Predicted molecular weight: 38 kDa.(Detection limit for recombinant Human Thrombopoietin: 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.)
Neut: Use at an assay dependent dilution. (Use at a concentration of 4.2-6.3 µg/ml to yield one-half maximal inhibition [ND50] of the biological activity of Human Thrombopoietin (5.00 ng/ml).)
Target
Function
Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.
Involvement in disease
Defects in THPO are a cause of essential thrombocythemia (ET) [MIM:187950]. ET is inherited as an autosomal dominant trait which is characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications.
Sequence similarities
Belongs to the EPO/TPO family.
Domain
Two-domain structure with an erythropoietin-like N-terminal and a Ser/Pro/Thr-rich C-terminal.
Cellular localization
Secreted.
Target information above from: UniProt accessionP40225
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- c mpl ligand antibody
- C-mpl ligand antibody
- Megakaryocyte colony stimulating factor antibody
see all
Database links
- Entrez Gene: 7066 Human
- Omim: 600044 Human
- SwissProt: P40225 Human
- Unigene: 1166 Human
References for Anti-Thrombopoietin antibody (ab84446)
ab84446 has not yet been referenced specifically in any publications.
Publishing research using ab84446? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"