Anti-Thrombospondin antibody [A4.1] (ab3131)

Thank you very much!I appreciate your helpfulness. However, the abcam reference number for my order is 775248 (attached to this email is the order form). I would like to replace the antibody with the Anti-Thrombospondin antibody [A6.1] (ab1823)

ANSWER:

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement for one vial of ab3131. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research.

We have ordered many antibodies from Abcam because we think that they work pretty good. We are performing experiments on extracellular matrices and antibodies against type I collagen, typa III collagen, type IV collagenn works greatly. However i have some concerns about hhe antibody Mouse monoclonal to Thrombospondin (ab3131). We've bought all this antibodies last years but after repeated assays i think that the thrombospondin one has some limits. We have used it in immunofluorescence on frosen sections, and effectively it stains something. However, after different western blotting in reducing and non reducing conditions, we noticed that it does not recognize a band of 450 KDa as stated in the datasheet but always bands lower than 60 KDa. Lysates were from murine megakaryocyte (well-documented producers of this protein). In the last year no abreviews have been posted on its internet page and this antibody has not yet been referenced in any publicautions. Could you please give some explanation?It seems that the clone A6.1 works better, is it possible to get a replacement with this clone?

ANSWER:

Thank you for your enquiry. Sorry to hear ab3131 is not working as expected. I would be happy to send you a replacement. If you could, please forward me the catalog number of the replacement antibody as well as the purchase order number or Abcam order reference number associated with the purchase of ab3131 so I can process your request.

Concerning molecular weight: On WB this antibody detects ~450 kDa non-reduced TSH. What band or bands are detected under reduced conditions?

ANSWER:

Thank you for your enquiry.

This antibody works only with non-reduced TSP (450 kDa). The MW of reduced TSP is 170-180 kDa.

Please do not hesitate to contact us if you have additional questions.

A customer wants to know if ab1823 and ab3131 are for Thrombospondin 1, 2 or both?

ANSWER:

Thank you for your enquiry.

Following contact with the source of this antiserum I have determined that ab3131 inhibits anti-angiogenic activity of TSP-1 by inhibiting its binding to TSP-receptor / CD36. I have been informed that it has specificity towards TSP1.

For more info. please see ref. by Prater CA, et. al. J Cell Biol. 1991, 112 (5): 1031-40.

ab1823 does cross react against TSP type 2.

I hope this information helps, please do not hesitate to contact me should you require further assistance.

Isotype is IgM - is it kappa or lambda?

ANSWER:

Ab3131 is IgM, kappa chain.

If you have any additional questions, please let us know.